UV-B辐射对拟南芥POD、SOD同工酶的影响  

Effect of UV-B Radiation on POD and SOD Isoenzyme of Arabidopsis thaliana

作　　者：李晓阳[1] 孙永星[1] 宋丽平[1] 韩榕[1] 

机构地区：[1]山西师范大学生命科学学院,山西临汾041004

出　　处：《西南农业学报》2011年第6期2143-2146,共4页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金(30671061);山西省自然科学基金(20081101)

摘　　要：选择哥伦比亚生态型(Columbia-0)的野生型拟南芥(Arabidopsis thaliana L.)为供试材料,研究了其POD、SOD同工酶对不同剂量UV-B胁迫的响应。结果显示,POD和SOD同工酶活性与UV-B辐射的剂量关系密切。POD、SOD同工酶活性在低剂量UV-B辐射时呈增加趋势,在中、高剂量UV-B辐射时活性则呈下降趋势。在UV-B胁迫下,POD同工酶酶带没有发生变化;SOD同工酶酶带在胁迫下,出现了新的酶带(SOD4、SOD5)。说明拟南芥在受到低剂量的UV-B胁迫下,可以通过提高自身的保护系统来抵御外界不良环境的影响;而受到较高剂量的UV-B胁迫时,会破坏植物的保护系统,造成不可逆的损伤。Ecotype of Columbia（Columbia-0） wild-type Arabidopsis（Arabidopsis thaliana L.） was used to study the respond of UV-B stress of different doses on its POD and SOD isoenzyme.The results showed that,POD and SOD isoenzyme activity was very closed to the dose of UV-B radiation.The activity of POD and SOD isoenzyme showed an increasing trend in the low dose of UV-B radiation,but in the medium and high doses of UV-B radiation,the activity was decreased.In the stress of UV-B,the enzyme belts of POD isozymes did not change,and new belts of SOD isozymes（SOD4,SOD5）was appeared.It was explained at low doses of UV-B stress,Arabidopsis could improve their own protection system to resist the adverse environmental impact from the outside world,but at high doses of UV-B stress,it would destroy the plant＇s protection system and cause irreversible damage.

关 键 词：UV-B 拟南芥 过氧化物酶 超氧化物歧化酶 

分 类 号：Q945.78[生物学—植物学]