5-aza-dc和TSA对广西陆川猪体细胞核移植的影响  

Effect of 5-aza-dc and TSA on Somatic Nuclear Transfer of Guangxi Luchuan-pig

作　　者：秦小娥[1] 王猛[1] 宋雪梅[1] 卢晟盛[1] 卢克焕[1] 

机构地区：[1]亚热带农业生物资源保护与利用国家重点实验室/广西大学动物繁殖研究所,广西南宁530004

出　　处：《西南农业学报》2011年第6期2372-2375,共4页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金资助项目(30860039);农业部"转基因生物新品种培育重大专项项目(2009ZX08010-023B);广西自然科学基金重点项目(2010GXNSFD013021);广西大学创新团队建设计划项目(2010)

摘　　要：为探讨甲基转移酶抑制剂5-aza-dc、去乙酰基转移酶抑制剂TSA对广西陆川猪体细胞核移植效率的影响,用5-aza-dc、TSA处理供体细胞和重构胚。结果显示,5-Aza-dc联合TSA处理供体细胞、重构胚以及连续处理供体细胞和重构胚,桑椹胚率均显著高于对照组(P<0.05),但各组间囊胚率差异不显著。说明5-aza-dc联合TSA处理供体细胞和克隆胚能提高猪克隆胚的发育能力,尽管差异不显著。To investigate the effect of 5-aza-20-deoxycytidine（5-aza-dC） and trichostatin A（TSA）on the somatic nuclear transfer of Guangxi Luchuan-pig,5-aza-dC and TSA were used to treat somatic cells and cloned embryos for decreasing the level of DNA methylation.The result showed that,the development rate of morular was significantly increased compared to control group（P0.05）with the treatment of donor cells,cloned embryos and continuous treatment of both donor cells and cloned embryos with a combination of 0.01 μM 5-aza-dC and 0.05 μM TSA,whereas the rate of blastocyte was not significantly different among the groups.It was concluded that： the development of cloned porcine embryos could be enhanced by the treatment of both donor cells and cloned embryos with a combination of 5-aza-dC and TSA.

关 键 词：猪 体细胞核移植 卵母细胞 5-AZA-DC TSA 

分 类 号：S828[农业科学—畜牧学]