凡纳滨对虾BTF3基因的克隆及表达分析  

Molecular Cloning and Expression Analysis of BTF3 in Litopenaeus vannamei

作　　者：殷勤[1,2] 彭金霞 崔亮 谢达祥 王志伟[2] 李奎[2] 陈晓汉 

机构地区：[1]广西壮族自治区水产研究所,广西水产遗传育种与健康养殖重点实验室,广西南宁530021 [2]中国农业科学院北京畜牧兽医研究所,北京100094

出　　处：《西南农业学报》2011年第6期2396-2400,共5页Southwest China Journal of Agricultural Sciences

基　　金：广西壮族自治区直属公益性科研院所基本科研业务费(2060302GXIF-2010-04);现代农业产业技术体系建设专项资金(编号:CARS-47);广西科学研究与技术开发计划(桂科攻10100007-2);国家科技支撑计划项目(2008BADB9B03)

摘　　要：通过电子克隆所得序列设计引物,采用RT-PCR方法首次克隆得到长655 bp的凡纳滨对虾通用转录因子3(Basic Tran-scription Factor 3,BTF3)基因序列,其中包括597 bp的完整开放阅读框,共编码198个氨基酸残基,推算分子量约45.79 kDa,理论等电点为4.93。氨基酸序列分析表明,BTF3基因编码的氨基酸序列中具有保守的NAC结构功能域,利用实时荧光定量PCR检测凡纳滨对虾不同组织的表达情况发现,该基因在凡纳滨对虾组织中广泛表达,其中肌肉组织表达量最高,在肠中表达量最低。A pair of primers were designed according to the sequence from electronic cloning,and a 655 bp cDNA of BTF3 in Litopenaeus vannamei was first obtained by RT-PCR.The cDNA contained a 597 bp open reading frame（ORF） encoding a 198 amino acids peptide.The molecular weight of BTF3 was 45.79 kDa,and its calculated isoelectric point was 4.93.Sequence alignment revealed that the amino acid sequence of BTF3 from different species were highly conserved and contained a NAC domain.Real-time PCR results showed that BTF3 mRNA could be detected in most of Litopenaeus vannamei tissues,and the highest expression level was observed in muscle,the lowest in intestine.

关 键 词：凡纳滨对虾 BTF3 基因克隆 表达分析 

分 类 号：S945.1[农业科学—水产养殖]