大孔吸附树脂法纯化苦豆子渣总黄酮工艺的研究  

Purification total flavonoids of Sophora alopecuroide residue using macroporous resins

作　　者：叶学军[1] 李力[2] 杨晋[2] 

机构地区：[1]宁夏专利服务中心,银川750001 [2]北方民族大学化学与化学工程学院,银川750021

出　　处：《食品科技》2012年第1期210-214,共5页Food Science and Technology

基　　金：宁夏回族自治区自然基金(NZ0845);国家民委重点开放实验室基金(2008SY15)

摘　　要：目的:考察5种大孔吸附树脂对苦豆子渣总黄酮的吸附分离性能。方法:以黄酮吸附量、解吸量为考察指标,采用静态和动态吸附分离法确定适合的大孔吸附树脂和纯化工艺条件。结果:AB-8型大孔吸附树脂对苦豆子渣总黄酮有良好的吸附分离性能,其最佳工艺为:最佳上样量为0.864mg/mL(树脂)、上样液流速为2BV/h、解吸液为95%乙醇、解吸液用量为4BV、解吸附流速为2BV/h。结论:AB-8可较好的吸附分离苦豆子渣总黄酮,纯化后黄酮纯度提高1倍以上。Five kinds of macroporous resins were used to adsorb and separate total flavonoids of Sophora alopecuroides residue. According to the amount of adsorption and desorption of flavonoid, static and dynamic adsorption separation methods were adopted to determine a suitable macroporous resin and purification procedure. The AB-8 macroporous adsorption resin showed good adsorption and separation performance. The purity of the purified flavonoids could be increased by 1 times when the best loading quantity was 0.864 mg/mL（resin）, sample flow rate was 2 BV/h, stripping liquid was 95% ethanol, the amount of stripping liquid was 4 BV and flow rate of stripping liquid was 2 BV/h.

关 键 词：大孔吸附树脂 苦豆子渣 总黄酮 分离纯化 

分 类 号：R284.2[医药卫生—中药学]