退白汤中补骨脂素和异补骨脂素含量测定方法研究  

Determination Method of Psoralen and Isopsoralen for Anti-vitiligo Decoction

作　　者：盛国荣[1] 

机构地区：[1]江苏省南通市皮肤病性病防治所,江苏南通226006

出　　处：《辽宁中医药大学学报》2012年第2期11-12,共2页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：南通市指令性社会发展科技计划资助项目(S2009047)

摘　　要：目的：建立双波长薄层扫描法测定退白汤中补骨脂素和异补骨脂素含量的方法。方法：选用补骨脂素λS=272nm，λR=305nm；异补骨脂素λS=275nm，λR=310nm；以甲苯-乙酸乙酯-甲酸（15：2：1）为展开剂，并在365m紫外灯下检视。结果：补骨脂素在0．27-1．62μg呈良好的线性关系（γ=0．9987），平均回收率99．46％，RSD=1．34％（n=5）；异补骨脂素在0．25-1．50μg呈良好的线性关系（γ=0．9992），平均回收率99．60％，RSD=1．37％（n=5）。结论：本法简便、快捷．可用于退白汤中补骨脂素和异补骨脂素的仓号测定．Objective ： To establish the determination method of dual-wavelength TLC for psoralen and isopsoralen in Anti-vitiligo decoction. Methods ： choose λ S=272nm, λR=305nm for determining psoralen, and choose λ S=275nm, λ R=310nm for determining isopsoralen. Developing agent：Toluene - ethyl acetateformic acid （15：2：1 ）, and determined under 35nm UV light. Results：Psoralen is under good linear relationship in the range of 0.27-1.62 μ g （ γ =0.9987 ）, and the average recovery ratio is 99.46%, RSD=1.34% （n=5）. Isopsoralen is under good linear relationship in the range of 0.25-1.5 μg （ γ=0.9992 ）, and the average recovery ratio is 99.60%, RSD=1.37% （n=5）.Conclusions：The method is simple and fast for the determination of psoralen and isopsoralen for Anti-vifiligo decoction.

关 键 词：退白汤 双波长薄层扫描法 补骨脂素 异补骨脂素 

分 类 号：R927.2[医药卫生—药学]