干旱胁迫对转IrrE基因甘蓝型油菜生理生化指标的影响  

Analysis of Physiological and Biochemical Indexes of IrrE-transgenic Brassica napus Plants under Drought Stress

作　　者：杨娟[1] 谢琳[1] 张新[1] 奉斌[1] 代其林[1] 王劲[1,2] 

机构地区：[1]西南科技大学极端环境生物资源利用实验室,四川绵阳621000 [2]中国农业科学院生物技术研究所,北京100084

出　　处：《作物杂志》2012年第1期26-30,共5页Crops

基　　金：转基因生物新品种培育重大专项(2009ZX08009-091B);国家自然科学基金(30871555);教育部新世纪优秀人才支持计划(NCET-08-0940);四川省教育厅青年基金项目(09ZA034);西南科技大学博士研究基金(11zx7104)

摘　　要：以转IrrE基因与非转基因甘蓝型油菜为材料,用10%(W/V)PEG6000(聚乙二醇)进行了干旱胁迫实验,比较研究了转IrrE基因与非转基因甘蓝型油菜幼苗的耐受性应答情况。结果表明:转基因油菜植株含水量下降趋势小于非转基因油菜植株;转基因油菜植株叶绿素含量和荧光参数F0的上升趋势均明显大于非转基因油菜植株;转基因油菜植株的Fv/Fm值和可溶性蛋白含量均大于非转基因油菜植株,但丙二醛(MDA)含量小于非转基因油菜植株。以上结果从侧面证实了:在干旱胁迫下,转IrrE基因油菜植株较非转基因植株有更强的耐受能力;IrrE基因作为一种转录调节因子,广泛参与了油菜幼苗对干旱胁迫的耐受性应答过程,增强了植株的耐旱能力。The tolerant responses of transgenic-IrrE and non-transgenic Brassica napus L. under drought stress induced by 10% （W/V） PEG 6000 （polyethylene glycol） was comparatively studied. The physiological index such as relative water content （RWC）, chlorophyll content, chlorophyll fluorescence parameters Fo, Fv/Fm, the malondialdehyde （MDA） content and soluble protein contents were investigated. The results showed that the relative water content （RWC） and Fv/Fm of transgenic-lrrE and non-transgenic B. napus leaves decreased gradually, but the malondialdehyde （MDA） content, soluble protein contents, chlorophyll content and chlorophyll fluorescence parameters F0 all increased gradually. In a conclusion,transgenic-IrrE B. napus showed higher resistance to drought than non-transgenic B. napus. IrrE gene, as a transcription factor, participated in the processes of tolerance response to drought stress broadly, could increase the tolerance to drought stress in transgenic-IrrE B. napus.

关 键 词：干旱胁迫 转IrrE基因甘蓝型油菜 叶绿素荧光 生理生化指标 

分 类 号：S565.4[农业科学—作物学]