肝细胞对白蛋白纳米粒的胞吞作用研究  

Uptake of Album Nanoparticles by Liver Cells

作　　者：金召英[1] 文志勇[1] 莫立乾[1] 毛萍[1] 杨西晓[1] 

机构地区：[1]南方医科大学南方医院药学部,广州510515

出　　处：《中国药学杂志》2012年第2期113-118,共6页Chinese Pharmaceutical Journal

基　　金：2010年度南方医院院长基金资助(2010B018)

摘　　要：目的研究L02细胞对白蛋白纳米粒的胞吞作用。方法采用钙黄绿素作为白蛋白纳米粒的荧光标记探针,用去溶剂化法制备钙黄绿素白蛋白纳米粒(calcein HSA-NPs),并考察粒径、体外释放、细胞摄取、胞内分布和细胞毒性。结果 CalceinHSA-NPs平均粒径为203.3 nm,体外释放96 h不到80%。白蛋白纳米粒的胞吞作用呈现时间、浓度、温度依赖性,可以显著提高水溶性小分子钙黄绿素的入胞能力(P<0.01)。Calcein HSA-NPs主要经clathrin介导的内吞途径和大胞饮入胞的,进入细胞后分布在细胞核外的胞内空间,主要集中在溶酶体内。白蛋白纳米载体对细胞基本无毒。结论所制备的calcein HSA-NPs可作为有效的载体来研究胞吞作用机制。OBJECTIVE To study the endoeytosis of albumin nanoparticles by L02 cells. METHODS Calcein human serum album nanoparticles （ calcein HSA-NPs） were prepared by a desolvation technique with caleein as a fluorescent probe. The panicle size distribution, in vitro release, cell uptake, intracellular drug distribution, and eytotoxieity were determined. RESULTS The prepared calcein HSA-NPs with an average diameters of 203.3 nm showed sustained release profile in vitro. The L02 cell uptake of nanopanicles showed time, concentration, and temperature dependency. HSA-NPs could significantly improve the transmembrane transport capacity of calcein as a water-soluble small molecule （ P 〈 0. 01 ）. Clathrin-mediated endocytosis and macropinoeytosis were the main routes by which calcein HSA-NPs entered into cells. After entering into cells, nanopartieles were located in the intracellular space outside the nucleus, mainly in the lysosomes. HSA-NPs had no eytotoxieity basically. CONCLUSION Caleein HSA-NPs can be used as an ef- fective vehicle to study the mechanism of endocytosis.

关 键 词：白蛋白纳米粒 胞吞作用 荧光强度 

分 类 号：R944[医药卫生—药剂学]