蔬菜大棚土壤有机提取物致基因组不稳定表型研究  

Phenotypes of Genomic Instability Induced by the Extractable Organic Matter from the Greenhouse Soil

作　　者：翟庆峰[1] 刘春兰 李媛媛[3] 邢杰[1] 邵丽军[1] 邱玉刚[1] 

机构地区：[1]潍坊医学院公共卫生学院,山东潍坊261042 [2]潍坊市奎文区卫生局卫生监督大队 [3]潍坊市妇幼保健院新生儿科

出　　处：《环境与健康杂志》2012年第1期46-48,共3页Journal of Environment and Health

基　　金：山东省医药卫生科技发展计划项目(2009QZ029)

摘　　要：目的研究温室土壤中有机提取物(extractable organic matter,EOM)的遗传毒性。方法采集并制备某蔬菜大棚土壤的EOM。40只健康10周龄清洁级昆明雄性小鼠随机分为5组:阴性对照(DMSO)组和低剂量(0.5 g/ml)、中剂量(1.5 g/ml)和高剂量(3 g/ml)EOM染毒组以及阳性对照(环磷酰胺,100 mg/kg体重腹腔注射,染毒2次)组,每组8只。采用灌胃方式进行染毒,每天1次,连续染毒4周。采用胞质分裂阻滞微核细胞组学试验检测有机提取物所致基因组不稳定表型和细胞毒性。结果随着有机提取物染毒浓度的增加,小鼠淋巴细胞微核细胞率、微核率、核质桥率、核芽率、细胞坏死率均呈上升趋势,双核细胞率和核分裂指数呈下降趋势,经相关分析,均P<0.05;而细胞凋亡率呈先升高后下降的趋势。结论温室土壤中有机提取物可以诱使小鼠外周血淋巴细胞产生基因组不稳定表型,并产生较强的细胞毒性,提示具有遗传毒性。Objective To study the phenotypes of genomic instability induced by the extractable organic matter（EOM） from the greenhouse soil. Methods EOM were obtained by the method of soxhlet extraction from greenhouse soil, and then were fed to mice via gavage （once a day for four consecutive weeks）.The chromosomal damage or instability status and the eytotoxicity were detected by the eytokinesis-block mieronucleus cytome （CBMN-Cyt） assay. Results There were significant differences between EOM exposure groups and controls for number of micronucleated cells,micronuelei, nueleoplasmic bridges,and nuclear buds. Those parameters increased significantly with EOM concentration. There were significant differences between exposure groups and control groups for Necr% ,with a dose-dependent manner. Conclusion EOM from the greenhouse soil can induce the phenotypes of genomic instability and the cytotoxicity in mice.

关 键 词：温室土壤 有机提取物 基因组不稳定 胞质分裂阻滞微核组学试验 

分 类 号：R994.6[医药卫生—毒理学]