氨溴索在大鼠胃内容物吸入性肺损伤中的抗炎作用  

Anti-inflammatory Roles of Ambroxol on RatsGastric Aspiration lung Injury

作　　者：张衍民[1] 贾晓民[1] 赵杰[1] 

机构地区：[1]徐州医学院第二附属医院呼吸内科,江苏徐州221006

出　　处：《临床肺科杂志》2012年第2期222-223,共2页Journal of Clinical Pulmonary Medicine

摘　　要：目的探讨氨溴索在大鼠胃内容物吸入后肺组织损伤中的抗炎作用。方法 30只健康SD雄性大鼠随机分为3组:对照组、损伤组、氨溴索组。肺组织湿重/干重比、谷胱甘肽过氧化物酶活性、超氧化物歧化酶活性的测定;ELISA法测定血清及肺泡灌洗液中TNF-αI、L-1βI、L-10含量;光镜下观察肺组织结构变化。结果与对照组比较,损伤组肺组织湿重/干重比增加(P<0.05)、谷胱甘肽过氧化物酶活性及超氧化物歧化酶活性均降低(P<0.05),血浆及肺泡灌洗液中肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、白细胞介素10(IL-10)含量增高(P<0.05);肺组织出现明显病理组织学损伤。与损伤组比较,氨溴索组肺组织湿重/干重比降低(P<0.05)、谷胱甘肽过氧化物酶活性及超氧化物歧化酶活性均增高(P<0.05),血浆及肺泡灌洗液中肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、白细胞介素10(IL-10)含量降低(P<0.05)。肺组织病理学损伤减轻。结论氨溴索可通过抗氧化应激及抑制炎性细胞因子释放在大鼠胃内容物吸入性肺损伤中发挥抗炎保护作用。Objective To investigate the anti - inflammatory roles of ambroxal on rats＇gastric aspiration lung injury. Methods Thirty Sprague-Dawley rats were randomly divided into three equal groups：Control group, injury group and ambroxol group. Wet-to-dry weight ratio（W/D） of lung, the glutathione peroxidase （GSH-Pxase） activity and the activity of superoxide dismutase （SOD） were measured ;Tumor necrosis factor-α （ TNF-α）, interlcukin-1β （ IL-1β ） and interlcukin-10 （ IL-10 ） of serum and bronchoalveolar lavage fluid （BALF） were examined by enzyme linked immunosorbent assay （ ELISA）. Lung pathological change was observed by microscope. Results Compared with the control group, TNF-α, IL-1 β, IL-10 of serum/BALF and W/D of lung in the injury group prominently increased （P 〈0. 05） , the activity of GSH-Pxase and SOD decreased（ P 〈 0. 05 ）. Compared with the injury group, TNF-ct, IL-1 [3, IL-10 of serum/ BALF and W/D of lung in the Ambroxol group prominently decreased （ P 〈 0. 05 ）, the activity of GSH-Pxase and SOD increased （ P 〈 0. 05 ）. The microscopic structure of the control group was normal and the pathological changes of the Ambroxol group were milder than those of the injury group. Conclusions Ambroxol has anti-inflammatory effects by restraining oxidative stress and the releasing of inflammatory cytokines on gastric aspiration lung injury.

关 键 词：氨溴索 肺损伤 抗炎作用 

分 类 号：R563.1[医药卫生—呼吸系统]