硝酸盐还原试验与反向斑点杂交试验联合检测结核分枝杆菌耐药性  

Joint detection of Mycobacterium tuberculosis drug resistance with Nitrate Reductase Assay and Reverse-dot Blot Hybridization

作　　者：潘建华[1] 石燕[1] 彭雪峰[1] 邓为之[1] 石国民[1] 向延根[1] 

机构地区：[1]长沙市中心医院,湖南长沙410004

出　　处：《临床肺科杂志》2012年第2期277-279,共3页Journal of Clinical Pulmonary Medicine

基　　金：湖南省卫生科技计划立项项目(No.2008074);国家十一.五科技中大专项(艾滋病和病毒性肝炎等重大传染病防治)(No.2009ZX10005-018)

摘　　要：目的联合检测结核分枝杆菌耐药性,以提高其灵敏度和特异性。方法对126株已知耐药浓度的结核分枝杆菌耐药株和敏感株分别采用硝酸盐还原试验(Nitrate Reductase Assay,NRA)和反向斑点杂交试验(reverse-dot blot hybridization,RBH),检测其异烟肼(INH)和对利福平(RFP)的耐药性,并将结果将与绝对浓度法结果相比较。结果硝酸盐还原试验和反向斑点杂交试验联合检测与绝对浓度法比较有高度的一致性(P>0.05),异烟肼灵敏度95.5%,特异性100%;利福平灵敏度100%,特异性98.1%。结论硝酸盐还原试验和反向斑点杂交试验联合检测可作为快速结核分枝杆菌耐药性试验方法。Objective To establish a joint detection method of Mycobacterium tuberculosis drug resistance to improve the sensitivity and specificity of resistance detection methods. Methods 126 resistant and sensitive strains of MTB were analyzed by Nitrate Reductase Assay （ NRA ） and Reverse-dot Blot Hybridization （RBH） for isoniazid （INH） and rifampicin （ RFP ）, respectively. The results were compared with absolute drug susceptibility tests. Results The joint detection was relatively high degree of consistency （ P 〉 0.05 ）, compared with the nitrate reductase assay, reverse-dot blot hybridization and the joint detection with absolute concentration method. The sensitivity of isoniazid （INH） and rifampicin （RPF） was 95.5%, 100%,and the specificity was 100%, 98. 1%. Conclusion The nitrate Reductase assay and reverse-dot blot hybridization can be used as fast joint detection of Mycobacterium tuberculosis drug resistance.

关 键 词：结核分枝杆菌 反向斑点杂交 药物耐受性 硝酸盐还原试验 聚合酶链反应 

分 类 号：R446.5[医药卫生—诊断学]