脱氧胆酸改性普鲁兰多糖纳米粒子制备与表征  

Preparation and characteristics of deoxycholic acid-modified pullulan nanoparticles

作　　者：史艳萍[1] 李学敏[2] 陈宝泉[1] 黄玉平[1] 张其清[2,3] 

机构地区：[1]天津理工大学化学化工学院,天津300384 [2]中国医学科学院生物医学工程研究所,天津市生物医学材料重点实验室,天津300192 [3]厦门大学医学院生物医学工程研究中心,福建厦门361005

出　　处：《功能材料》2012年第2期181-184,共4页Journal of Functional Materials

基　　金：国家重大科学研究计划资助项目(2006CB933300);国家教育部博士点基金资助项目(20101106110042)

摘　　要：通过酯化反应将脱氧胆酸偶联于普鲁兰多糖骨架形成具有两亲性的普鲁兰多糖衍生物(DP),采用纳米沉淀法制备纳米粒子(DPNs),考察制备条件对纳米粒子性质影响,为进一步将其作为药物载体的研究提供基础。衍生物DP结构通过FT-IR和1 H NMR表征,DPNs经透射电镜、动态光散射仪和zeta电位仪表征检测。获得不同取代度脱氧胆酸改性普鲁兰多糖衍生物,制备得到的纳米粒子呈球形,表面光滑规整,平均粒径100～300nm,zeta电位在-20mV左右。脱氧胆酸改性普鲁兰多糖衍生物通过纳米沉淀法能制备出纳米粒子,颗粒性质受制备条件影响。To synthesize deoxycholic acid-modified pullulan derivation（DP） by coupling deoxycholic acid to pullulan and prepare nanoparticles（DPNs） by nanoprecipitation.The effects of different parameters on the nanoparticles formation were investigated for further research of the DP as targeting anti-tumor drug carriers.FT-IR spectroscopy（Bio-Rad Merlin） and 1H NMR（INOVA 500Hz） were used to confirm the synthesis of the DP.The physicochemical properties of the nanoparticles were characterized using transmission electron microscopy（TEM）,dynamic light scattering（DLS） and zeta potential.DP with different degree of substitution were synthesized.The TEM demonstrated that the shape of nanoparticles was spherical with smooth surface.The mean diameter was in the range of 100-300 nm and zeta potential was-20mV.Deoxycholic acid-modified pullulan was formed nanoparticles by nanoprecipitation.Their physicochemical properties were affected by parameter of preparation.

关 键 词：脱氧胆酸 普鲁兰多糖 纳米粒子 纳米沉淀法 自组装 

分 类 号：TQ050.425[化学工程]