1460例男性患者性传播疾病病原体荧光定量聚合酶链反应检测分析  

Analysis of FQ-PCR detection on sexually transmitted disease pathogens among 1460 male patients

作　　者：师志云[1] 赵志军[1] 赵颖[1] 马俊[1] 贾伟[1] 魏军[1] 

机构地区：[1]宁夏医科大学总医院医学实验中心,银川750004

出　　处：《检验医学与临床》2012年第1期38-39,共2页Laboratory Medicine and Clinic

摘　　要：目的了解宁夏某医院门诊男性患者淋球菌(NGH)、沙眼衣原体(CT)、解脲支原体(Uu)、单纯疱疹病毒(HSV-Ⅱ)、人乳头瘤病毒(HPV6,11)5种性传播疾病(STD)病原体的检出情况,为临床预防提供依据。方法采用实时荧光定量聚合酶链反应法对1 460例患者进行NGH、CT、Uu、HSV-Ⅱ、HPV6,11病原体DNA定量检测。结果 STD病原体总阳性率为20.34%,HPV6,11、HSVⅡ、Uu、CT和NGH阳性率分别为69.57%、30.00%、24.21%、19.15%和6.32%;21～40岁年龄段患者占86.20%。结论 HPV6,11为男性STD的主要感染因素,应引起全社会的高度重视。Objective To learn about the infection status of 5 common sexually transmitted disease（STD） pathogens of neisseria gonorrhoeae（NGH）,chlamydia trachomatis（CT）,ureaplasma urealyticum（Uu）,herpes simplex virus（HSV-Ⅱ）,human papilloma virus（HPV6,11） among male outpatients in our hospital and to provide evidence for the prevention and treatment of these STDs.Methods Samples were taken routinely from 1 460 outpatients to detect NGH,CT,Uu,HSV-Ⅱ and HPV6,11 by FQ-PCR.Results Total positive rate was 20.34%.The positive rates of HPV6,11,HSVⅡ,Uu,CT and NGH were 69.57%,30.00%,24.21%,19.15% and 6.32%.The patients aged 21-40 years accounted for 86.20%.Conclusion HPV6,11 pathogen is the main infectious factor for STD,and should be paid attention to by the whole society.

关 键 词：性传播疾病 淋球菌 沙眼衣原体 解脲脲原体 单纯疱疹病毒 人乳头瘤病毒 荧光定量聚合酶链反应 

分 类 号：R759[医药卫生—皮肤病学与性病学]