胶原蛋白-壳聚糖复合纳米纤维膜用于皮肤缺损的修复  

Repair of skin defect with collagen-chitosan compound nanofiber membrane

作　　者：余丕军[1,2] 王露萍[3] 郭妤[3] 陈炜[3] 莫秀梅[4] 

机构地区：[1]江苏大学临床医学院,江苏省镇江市212013 [2]上海交通大学医学院附属第九人民医院麻醉科,上海市200011 [3]上海市第八人民医院,上海市200235 [4]东华大学化工与生物工程学院,上海市201620

出　　处：《中国组织工程研究与临床康复》2011年第51期9561-9564,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家科技部863科研基金资助项目(2008AA03Z305)~~

摘　　要：背景:体外实验证实:胶原蛋白-壳聚糖(80:20)复合纳米纤维膜具有优异的生物力学性能、生物相容性和生物可降解性。但目前缺乏此材料在体内方面的研究。目的:观察胶原蛋白-壳聚糖复合纳米纤维膜修复SD大鼠背部全层皮肤缺损创面的作用。方法:30只SD大鼠制备背部全层皮肤缺损创面后随机抽签法分为2组,实验组用胶原蛋白-壳聚糖(80:20)复合纳米纤维膜覆盖后,用油纱及干纱布包扎并创缘外缝线打包固定;对照组仅用油纱及干纱布包扎并创缘外打包固定。结果与结论:修复后14d实验组创面已基本对合,干痂;苏木精-伊红染色显示创面毛细血管数量减少,纤维含量增多。对照组创面对合不整齐,创面较实验组大;苏木精-伊红染色显示毛细血管数量较多,扩张明显,大量炎性细胞浸润。提示胶原蛋白-壳聚糖复合纳米纤维膜较普通纱布敷料能更好的促进创伤修复、愈合。BACKGROUND：Studies in vitro have confirmed that collagen-chitosan（80：20） compound nanofiber membranes have excellent mechanical properties,biocompatibility and biodegradability.However,there are few researches in vivo about the materials.OBJECTIVE：To observe the effect of collagen-chitosan compound nanofiber membranes on repair of full-thickness skin defect on the back of Sprague Dawley（SD） rats. METHODS：Thirty SD rats with full-thickness skin defects on the back were divided into two groups randomly by lottery method.SD rats treated with collogen-chitosan（80：20） compound nanofiber membrane and oily gauzes on the defects were taken as the experimental group,while those treated with oily gauzes only as the control group.The defects of all rats were fixed with dry gauze bandage at the margin of outer packaging.RESULTS AND CONCLUSION：The wounds healed basically in the experimental group at 14 days after the repair;hematoxylin-eosin staining showed that the numbers of capillaries were reduced and the content of fibers was increased.While in the control group,wound healed irregularly,and the wound was larger than that in the experimental group;hematoxylin-eosin staining showed that there were more obvious dilated capillaries and a large number of inflammatory cells.The above results indicated that collagen-chitosan compound nanofiber membrane is superior to ordinary gauze in promoting the tissue repair and wound healing of skin defects.

关 键 词：胶原蛋白 壳聚糖 复合纳米纤维膜 皮肤缺损 创伤修复 

分 类 号：R318[医药卫生—生物医学工程]