(188)~Re-碘化油-羧甲基壳聚糖-纳米微粒在荷S180肉瘤小鼠体内分布与代谢  

In vivo distribution and metabolism of ^(188)Re-iodized oil-carboxymethyl chitosan-nanoparticles in the S180 tumor-bearing mice

作　　者：王洪震[1] 贾正平[1] 郝彦明[1] 钱荣勋[1] 董启榕[2] 徐又佳[2] 

机构地区：[1]昆山市第一人民医院,江苏省昆山市215300 [2]苏州大学附属二院,江苏省苏州市215000

出　　处：《中国组织工程研究与临床康复》2011年第51期9603-9607,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:188Re标记的放射性药物在体内发生Re核索脱落也不会对人体造成严重的辐射损伤。188Re-碘化油有可能成为一种具有临床应用价值的内照射治疗肿瘤的药物。目的:研究羧甲基壳聚糖载药纳米微球的制备及在荷S180肉瘤小鼠体内分布及代谢。方法:将188Re-碘化油通过羧甲基壳聚糖纳米微球包裹,将其注射于荷S180肉瘤小鼠体内,通过SPECT法观察188Re-碘化油-羧甲基壳聚糖-纳米微粒在荷瘤鼠中的显像。结果与结论:采用羧甲基壳聚糖-纳米微粒对188Re-碘化油标记率达(94.9±0.2)%;肝、肾是188Re-碘化油-羧甲基壳聚糖-纳米微粒的主要分布器官;骨、肌肉、小肠等脏器摄取较少,且随着时间的延长而下降;脑内未测得放射性,各比值随着时间的延长而有增加的趋势,分别在注射显像剂后6～10h达到峰值。说明羧甲基壳聚糖-纳米微粒对188Re的包裹效果良好;188Re-碘化油-羧甲基壳聚糖-纳米微粒在正常骨、肌肉、小肠基本无摄取,但48h浓聚于肉瘤组织内,肿瘤与脾、胃、肠、股骨、肌肉、脂肪组织放射性比值高。BACKGROUND：188Re labeled radiopharmaceuticals in body occur Re nuclear cable off which will not cause serious radiation damage of the body.188Re-iodized oil may become a kind of clinical application of drugs within the radiation treatment of cancer.OBJECTIVE：To study preparation of drug-loaded nano-carboxymethyl chitosan microspheres and in vivo distribution and metabolism in the S180 tumor-bearing mice.METHODS：188Re-lipiodol was wrapped by carboxymethyl chitosan nanoparticles,the wrapped 188Re-lipiodol was injected into the mice bearing S180 sarcoma.Imaging of 188Re-iodized oil-carboxymethyl chitosan-nanoparticles in the tumor-bearing mice was observed by single photon emission computed tomography. RESULTS AND CONCLUSION：Labeling rate of 188Re-lipiodol which labeled by carboxymethyl chitosan-nanoparticles was（94.9±0.2）%.188Re-iodized oil-carboxymethyl chitosan was distributed in liver and kidney;and the intake decreased with time in bone,muscle,small intestine and other organs.Radiation was not measured in brain,and the ratio increased with time,and imaging agent reached to peak at 6 to10 hours after injecting.It is indicated that wrapped effect of 188Re by carboxymethyl chitosan-nanoparticles package is well.188Re-iodized oil-carboxymethyl chitosan-Nanoparticles in normal bone,muscle and small intestine is almost not uptaked,but 188Re-iodized oil-carboxymethyl chitosan-Nanoparticles can uptake in sarcoma tissue within 48 hours and the radioactivity ratio of the tumor and spleen,stomach,intestine,femur,muscle and adipose tissue is high.

关 键 词：羧甲基壳聚糖 纳米微球 肉瘤小鼠 体内分布 代谢 

分 类 号：R318[医药卫生—生物医学工程]