Kinectin-麦芽糖结合蛋白融合蛋白致敏树突状细胞对T细胞分化格局的改变  

Effect of sensitized dendritic cells with kinectin-maltose-binding protein on T cell differentiation activity

作　　者：赵飞兰[1] 晁耐霞[1] 李日伦[1] 曾庆堂[1] 黄天明[1] 莫发荣[1] 肖飞[1] 罗国容[1] 

机构地区：[1]广西医科大学组胚教研室,广西壮族自治区南宁市530021

出　　处：《中国组织工程研究与临床康复》2011年第53期9991-9995,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金(81060168/H1602);课题名称:肿瘤相关抗原GCF2在肝癌发生中的作用机理研究;广西自然科学基金重大项目(2010GXNSFD013048);课题名称:SEREX筛选的肝癌相关抗原的鉴定及临床应用研究;广西中医学院院级课题(P2008009);课题名称:动力素kinectin质粒的构建;表达及致敏DC抗肝癌活性研究~~

摘　　要：背景:Kinectin作为树突状细胞瘤苗可运用于肝癌临床免疫治疗。目的:检测kinectin-麦芽糖结合蛋白融合蛋白致敏的树突状细胞刺激T细胞亚群的分化情况。方法:运用免疫组化法检测kinectin-麦芽糖结合蛋白孵育的树突状细胞刺激增殖的T细胞中CD4+、CD8+T细胞亚群活化情况,同时设立麦芽糖结合蛋白组、非致敏树突状细胞组及普通培养T细胞组作为比较。结果与结论:Kinectin-麦芽糖结合蛋白致敏树突状细胞刺激的T细胞组CD8+T细胞明显高于麦芽糖结合蛋白孵育树突状细胞刺激的T细胞组、非致敏树突状细胞刺激组及普通培养T细胞组(P<0.05);各组CD4值之间、CD4/CD8值之间比较,差异无显著性意义(P>0.05)。经Kinectin-麦芽糖结合蛋白融合蛋白致敏的树突状细胞能有效刺激自体CD8+T淋巴细胞的增殖,Kinectin-麦芽糖结合蛋白作为肝癌相关抗原可通过致敏树突状细胞发挥较明显的抗肿瘤功能。BACKGROUND： Kinectin, as a dendritic cell tumor seed, can be used for clinical immunotherapy of hepatocellular carcinoma. OBJECTIVE： To evaluate the effect of kinectin-maltose-binding protein （MBP）-sensitized dendritic cells （DC） on autologous T cell differentiation activity. METHODS： The proliferation of CD4＋, CD8＋ T cell subset stimulated by DC incubated with kinectin-MBP was detected by immunohistochemistry. Simultaneously, kinectin-MBP, non-sensitized DC, and commonly cultured T cells groups were set for comparison. RESULTS AND CONCLUSION： The number of CD8＋ T cells was increased significantly in the kinectin-MBP-DC-T cell group than in the kinectin-MBP, non-sensitized DC, and commonly cultured T cells groups （P 〈 0.05）. There was no significant difference in the number of CD＋ T cells and the ratio of CD4 to CD8 among the groups （P 〉 0.05）. The results showed that kinectin-MBP-sensitized DC can effectively stimulate the proliferation of autologous CD8＋ T cells and kinectin-MBP as a hepatocellular carcinoma-related antigen can exert obvious anti-tumor function via the sensitized DC.

关 键 词：kinectin-MBP融合蛋白 树突状细胞 肝细胞癌 T细胞亚群 CD8 

分 类 号：R318[医药卫生—生物医学工程]