β-受体阻断药物对大型蚤的毒性研究  

Toxicity of β-adrenergic receptor blockers to Daphnia(Daphnia magna)

作　　者：施嘉琛[1,2] 尚楠[3] 张晶[1,2] 邵兵[1,2] 

机构地区：[1]北京市疾病预防控制中心,北京100013 [2]北京市预防医学研究中心 [3]北京中医药大学东方医院药学部

出　　处：《毒理学杂志》2011年第6期418-421,共4页Journal of Toxicology

基　　金：国家自然科学基金(20807007)

摘　　要：目的研究五种常用β-受体阻断药物(比索洛尔、普萘洛尔、索他洛尔、阿替洛尔和美托洛尔)对大型蚤(Daphnia magna)的毒理学效应。方法根据OECD操作规程,开展5种目标药物对大型蚤的48 h毒性实验,获得各药物的半数抑制浓度EC50。再对48 h毒性效应较强的两种药物开展21d毒性实验,考察相关生理学指标的变化。结果 5种药物的48 h半数抑制浓度EC50为6～169 mg/L。其毒性顺序为普萘洛尔>比索洛尔>索他洛尔>阿替洛尔>美托洛尔。普萘洛尔和比索洛尔的21d毒性试验结果表明两种药物对大型蚤的生理指标均存在毒理学效应,包括怀卵和产蚤时间延迟、产蚤数减少及体长缩短等。结论β-受体阻断药物对大型蚤(Daphnia magna)存在毒理学效应,加强该类药物的使用和管理具有现实的生态毒理学意义。Objective To investigate the toxicity of 5 β-adrenergic receptor blockers （bisoprolol, propranolol, sotalol, atenolol and metoprolol） to crustacean Daphnia magna. Methods The EC50 of five β-adrenergic receptor blockers were evaluated on the 48 h toxicity experiments according to the standard protocol established by OECD. The two strongest toxic blockers were chosen to study the 21 day toxicity to physiological change of daphnia. Results The EC50 of five β-adrenergic receptor blockers were 6-169 mg/L. The toxicities （48 h ECso value, mg/L） in decreasing order were propranolol, bisoprolol, sotalol, atenolol and metoprolol. The 21 day toxicity study showed both propranolol and bisoprolol owning toxic effects to daphnia. The physiological change include the delay of first pregnancy and first brood, decrease of the number of broods per female and shorten of body length. Conclusion Since the β-adrenergic receptor blockers were toxicity to Daphnia magna which was a commonly used test animal in aquatic toxicology, the management of these medicines should be concern on the ecotoxicology.

关 键 词：β-受体阻断药物 大型蚤 48 h毒性 21 d毒性 

分 类 号：R994.6[医药卫生—毒理学]