人胸腺组织原代培养细胞与人类免疫缺陷病毒间的相互作用  

Interaction between primary cultured human thymocytes and human immunodeficiency virus

作　　者：高楠竣 陈英杰[2] 李雪涛[3] 孔维云[3] 周轶平[4] 

机构地区：[1]云南省玉溪市第三人民医院儿科,云南省玉溪市653100 [2]昆明医学院人体解剖学教研室,云南省昆明市650031 [3]云南省第二人民医院骨科,云南省昆明市650051 [4]昆明医学院药学院暨重点实验室,云南省昆明市650031

出　　处：《中国组织工程研究与临床康复》2011年第50期9344-9347,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：云南省科委基金资助项目;云南省科技厅-昆明医学院联合专项基金资助项目(98C014Q ;2007C0044R;2009CD188);云南省高校药学重点实验室创新平台建设专项基金(6010902009-2010ZL004);云南省教育厅科学研究基金立项资助项目(2010Y191 ;07Z10199)~~

摘　　要：背景:人类免疫缺陷病毒能够结合人胸腺的细胞CD4受体,从而导致CD4细胞活性降低。目的:观察人胚胎胸腺组织原代培养与人类免疫缺陷病毒相互作用关系。方法:取人胎胸腺组织进行原代细胞培养,取胸腺的细胞与人类免疫缺陷病毒1ⅢB混合培养设为实验组,设置不加人类免疫缺陷病毒培养的胸腺的细胞为对照组。结果与结论:透射电镜观察显示,培养40h,人类免疫缺陷病毒诱导的胸腺的细胞内即可发现大量艾滋病病毒颗粒。MTT法和免疫组织化学染色检测结果显示,培养40h^22d,HIV诱导胸腺的细胞吸光度值均显著低于对照组(r=0.9733,P<0.05),人类免疫缺陷病毒诱导胸腺的细胞膜上和细胞浆Fas-L阳性表达率均升高(P<0.05)。说明人类免疫缺陷病毒可致原代培养的人胚胎胸腺的细胞活性降低。BACKGROUND： Human immunodeficiency virus binds to CD4 receptors in human thymocytes, and thus causes activity decreasing in CD4 cells. OBJECTIVE： To explore the relationship between human embryo thymocytes and human immunodeficiency virus. METHODS： Primary cultured human embryo thymocytes were divided into experiment group and control group. Experimental thymocytes were mixed cultured with human immunodeficiency virus 1 [H B. Control thymocytes were cultured without human immunodeficiency virus. RESULTS AND CONCLUSION： According to transmission electron microscopy, massive human immunodeficiency virus particles emerged in thymocytes treated with human immunodeficiency virus at the 40th hour after cultivation. According to MIF assay and immunohistochemical staining, the absorbance in thymecytes treated with human immunodeficiency virus was significantly lower than that in the control thymocytes at the 40th hour to the 2ndday after cultivation （r=0.9733, P 〈 0,05）. The Fas-L positive rate of the experiment thymocytes treated with human immunodeficiency virus increased on cell membranes and in cytoplasm （P 〈 0.05）. These findings indicate that human immunodeficiency virus decreases the cell activity in primary cultured human embryo thymocytes.

关 键 词：人类免疫缺陷病毒 胸腺 原代培养:细胞活性 组织构建 

分 类 号：R318[医药卫生—生物医学工程]