持续静水压对细胞增殖的影响  

Effect of continuous static hydrostatic pressure on cell proliferation

作　　者：郭紫芬[1] 冯勇[1] 李凯[1] 廖端芳[1] 

机构地区：[1]南华大学药物药理研究所,湖南省衡阳市421001

出　　处：《中国组织工程研究与临床康复》2011年第50期9353-9355,共3页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家863专题项目(2008AA02Z436);国家自然科学基金(81102516);湖南省研究生创新项目(CX2009B191)~~

摘　　要：背景:血管平滑肌细胞在静水压替代血清培养的条件下能良好生长。目的:探讨无血清水平状态下,持续性静水压对细胞增殖的影响。方法:采用自行研制的压力可调细胞培养箱在0,12,16,20,24和28kPa条件下培养血管平滑肌细胞A10与血管内皮细胞HUVEC-12。结果与结论:A10细胞在16kPa压力的条件下增殖最明显,活性最强;HUVEC-12细胞在20kPa压力的条件下增殖最明显,活性最强。说明一定范围内的静水压可促进体外培养的A10与HUVEC-12细胞增殖、使其活性增加。BACKGROUND： Vascular smooth muscle cells grow well in culture condition using static hydrostatic pressure instead of serum OBJECTIVE： To investigate the effect of continuous static hydrostatic pressure on the proliferation of vascular smooth muscle cells. IETHODS： Vascular smooth muscle cell A10 and vascular endothelial cell HUVEC-12 were cultured in a self-developed pressure adjustable incubator, under different pressures of 0, 12, 16, 20, 24 and 28 kPa respectively. RESULTS AND CONCLUSION： The most obvious proliferation of A10 cell was detected under the pressure of 16 kPa, with greatest cell activity. And the most obvious proliferation of HUVEC-12 cell was under 20 kPa pressure, with greatest cell activity It confirms that limited hydrostatic pressure can promote the proliferation of A10 and HUVEC-12 and increase the cell activity.

关 键 词：高静水压 无血清细胞培养 血管内皮细胞:血管平滑肌细胞 增殖 

分 类 号：R318[医药卫生—生物医学工程]