皮质脊髓束半横断损伤模型大鼠皮质脊髓束的超微结构改变  

Ultrastructural changes of the corticospinal tract in rats after corticospinal tract semitransection

作　　者：刘苏[1] 沈光宇[1] 吕广明[2] 

机构地区：[1]南通大学附属医院康复科,江苏省南通市226001 [2]南通大学医学院人体解剖学系,江苏省神经再生重点实验室,江苏省南通市226001

出　　处：《中国组织工程研究与临床康复》2011年第50期9369-9372,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：江苏高校优势学科建设工程资助项目~~

摘　　要：背景:有关皮质脊髓束受损后损伤附近及远处超微结构的整体变化的报道甚少。目的:观察大鼠皮质脊髓束半横断损伤后皮质脊髓束超微结构的变化。方法:选择性切割SD大鼠延髓左侧锥体,建立皮质脊髓束半横断损伤模型,于造模后4,14,28d取受损皮质脊髓束,制备电镜标本。结果与结论:透射电镜观察发现受损皮质脊髓束髓鞘和轴索发生肿胀,形态不规则。随着时间的延长,溃变进行性加重,受损皮质脊髓束主要表现为髓鞘破坏、溶解及轴索脱髓鞘病变、胞浆浓缩、细胞器增多及空泡样变性。脊髓颈、胸、腰段受损皮质脊髓束的溃变比损伤部位严重。BACKGROUND： Researches on the overall ultrastructural changes of the body parts near and distant from the corticospinal trac lesion are rarely reported. OBJECTIVE： To investigate the ultrastructural changes of corticospinal tract in rats after corticospinal tract semitransection. METHODS： Rat left vertebral body of medulla was selectively cleavaged to construct corticospinal tract semitransection model. Electron microscopy was utilized to determine the degeneration of the corticospinal tract on the 4th, 14th and 28th days after corticospinal tract semitransection. RESULTS AND CONCLUSION： According to transmission electron microscope observation, the myelin sheath and axons of lesioned corticospinal tract were swelling and irregular in shape. Subsequently, during the extended periods, the ultrastructural changes were myelinoclasis, dissolvation in myelin sheath, and demyelination, shrink of cytoplasm, vacuolar degeneration, increase of organelles in axons progressively. These changes of lesioned cortlcospinal tract at cervical, thoracic, lumbar segments of spinal cord were more serious than those closed to lesion site.

关 键 词：皮质脊髓束 超微结构 半横断损伤 溃变 髓鞘 

分 类 号：R318[医药卫生—生物医学工程]