复发性阿弗他溃疡模型大鼠细胞免疫状态与黄芪多糖的干预  

Effect of astragalus polysaccharide on cellular immunity of rats with recurrent aphthous ulcer

作　　者：王雪梅[1] 薄磊[1] 李欣 张菊梅[1] 余占海[1] 

机构地区：[1]兰州大学口腔医学院,甘肃省兰州市730000 [2]解放军兰州军区空军机关门诊医院,甘肃省兰州市730000

出　　处：《中国组织工程研究与临床康复》2011年第50期9462-9465,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:复发性阿弗他溃疡病因复杂,可能与免疫调节失衡有关。目的:探讨细胞免疫异常与复发性阿弗他溃疡发病的关系及黄芪多糖对复发性阿弗他溃疡模型大鼠的免疫调节作用。方法:采用免疫法建立SD大鼠复发性阿弗他溃疡模型,造模成功后分别给予100,200,300mg/kg黄芪多糖或生理盐水灌胃,以正常饲养的大鼠作为对照。用流式细胞仪检测各组SD大鼠外周血T淋巴细胞亚群CD4+、CD8+细胞数量及比值。结果与结论:流式细胞仪检测结果显示复发性阿弗他溃疡大鼠外周血CD4+细胞数量、CD4+/CD8+比值显著降低,而CD8+细胞数量明显升高(P<0.05)。连续灌胃100,200,300mg/kg黄芪多糖20d后,复发性阿弗他溃疡大鼠外周血CD4+细胞数量及CD4+/CD8+比值均有所回升,CD8+细胞数量降低,以300mg/kg黄芪多糖效果最明显。说明复发性阿弗他溃疡发生与T淋巴细胞亚群不平衡有关,黄芪多糖可剂量依赖性改善复发性阿弗他溃疡大鼠的异常免疫状态。BACKGROUND： Recurrent aphthous ulcer is caused by complicated factors. It may be related to the imbalance of immune regulation. OBJECTIVE： To investigate the relationship between the abnormal cellular immune and the pathogenesis of recurrent aphthous ulcer and to explore the immune regulation of astragalus polysaccharide in rats with recurrent aphthous ulcer. METHODS： Immunization method was used to establish recurrent aphthous ulcer model in SD rats. Rats were treated with 100, 200 and 300 mg/kg astragalus polysaccharide or saline by gavage after model establishment. Rats receiving normal feeding were used as control. The values of peripheral blood T lymphocyte subsets CD4＊, CD8~ and CD4＊/CD8~ in SD rats of each group were measured by flow cytometry. RESULTS AND CONCLUSION： According to flow cytometry, the values of peripheral blood CD4~ and CD4＋/CD8~ decreased significantly in rats with recurrent aphthous ulcer, while the value of CD8~ increased significantly （P 〈 0.05）. On the 20th day after treated with 100, 200 and 300 mg/kg astragalus polysaccharide by gavage, the values of peripheral blood CD4~ and CD4~/CD8＋ partially recovered in rats with recurrent aphthous ulcer, expecially in the rats treated with 300 mg/kg astragalus polysaccharide. It demonstrates that the unbalance of T-lymphocyte is related to the pathogenesis of recurrent aphthous ulcer. Astragalus polysaccharide can dose-dependently improve the abnormal immune state in rats with recurrent aphthous ulcer.

关 键 词：复发性阿弗他溃疡 黄芪多糖 SD大鼠 T淋巴细胞亚群 免疫 

分 类 号：R318[医药卫生—生物医学工程]