Caspase-3激活的DNA酶在缺血再灌注神经元损伤中的表达  

Expression of caspase-activated DNase in the neuronal injury after ischemia-reperfusion

作　　者：运志媛[1] 金永华[2] 蔡文进[1] 

机构地区：[1]哈尔滨医科大学附属第三医院经纬医院内科,黑龙江哈尔滨150010 [2]哈尔滨医科大学附属第二医院神经内科,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2011年第6期537-540,共4页Journal of Harbin Medical University

基　　金：黑龙江省科委攻关课题(GC05C40601)

摘　　要：目的探讨大鼠局灶性脑缺血再灌注后缺血半暗带半胱氨酸蛋白酶-3(Caspase-3)激活的DNA酶(caspase-activated Dnas,CAD)基因、CAD蛋白的表达及Caspase-3抑制剂对缺血神经元的保护作用及其机制。方法线栓法制备大鼠大脑中动脉闭塞(MCAO)及再通模型,利用RT-PCR与Westernblot技术检测MCAO再灌注后6 h、12 h、24 h、48 h、72 h时缺血半暗带CAD基因及蛋白的表达。结果脑缺血再灌注后出现CADmRNA表达上调及CAD蛋白表达增加。应用Caspase-3抑制剂后(Ac-DEVD-CHO),CADmRNA及CAD蛋白表达均下降。结论 Ac-DEVD-CHO可能通过减少缺血再灌注神经元CAD基因及蛋白表达,对缺血性神经元起到保护作用。Objective To investigate the expression of caspase-activated DNase （CAD） gene and protein in ischemic penumbra after rat focal ischemic middle cerebral artery reperfusion and the pbssible mechanisms of Caspase-3 inhibitor against neuronal injury. Methods The middle cerebral artery of rats was occluded for 2 h by an intraluminal filament, and reperfusion was carried out for 6 h, 12 h, 24 h, 48 h and 72 h. The expression of CAD gene and protein in different time was detected by RT-PCT＇and Western blot. Results CAD mRNA and CAD pro- tein increased after ischemic-reperfusion and Caspase-3 inhibitor decreased the expression of CAD mRNA and CAD protein. Conclusion Caspase-3 inhibitor could protect neurons against injury about reperfusion of cerebral ischemia by reducing the expression of CAD gene and protein.

关 键 词：脑缺血再灌注 半胱氨酸蛋白酶-3 半胱氨酸蛋白酶激活的DNA酶 半胱氨酸蛋白酶-3抑制剂 

分 类 号：R332[医药卫生—人体生理学] R743[医药卫生—基础医学]