草仙乙肝胶囊含药血清诱导大鼠肝星状细胞凋亡及机制研究  

Effect of serum containing Caoxianyigan capsule on apoptosis in rat hepatic stellate cells-T6 and its mechanism

作　　者：高杰[1] 许春海[1] 梁明[1] 李树臣[1] 

机构地区：[1]哈尔滨医科大学附属第二医院感染病科,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2011年第6期550-552,共3页Journal of Harbin Medical University

基　　金：黑龙江省教育厅科学技术研究项目(11551248)

摘　　要：目的观察草仙乙肝胶囊含药血清对肝星状细胞株HSC-T6凋亡的影响及调控机制。方法制备草仙乙肝含药血清,MTT法和流式细胞仪检测草仙乙肝药物血清对HSC-T6细胞增殖和诱导凋亡影响;采用Western blot检测Bax、Caspase-3表达。结果草仙乙肝药物血清与对照组比较可抑制HSC-T6细胞的增殖(P<0.05);而HSC-T6细胞凋亡数明显增加(P<0.05),Western blot分析显示药物血清作用下,HSC-T6线粒体Bax、细胞质Caspase-3表达上调。结论草仙乙肝胶囊含药血清可显著抑制HSC-T6细胞的增殖,并诱导其凋亡。其可能是通过影响Bax蛋白异常表达来起到对HSC-T6细胞的抑制增殖及促凋亡作用。Objective To investigate the effect and possible mechanism of Caoxianyigan cap- sule on apoptosis in cultured rat hepatic stellate cells （line, HSC ） HSC-T6. Methods First sera containing Caoxianyigan capsule were produced, the effect of drug-contained sera on the prolife-ration of HSC-T6 cells was detected by MTT assay and apoptosis rate was detected by flow cytometry. Western blot analysis was performed to determine the Bax. Caspase-3 in the HSC-T6 cells homogenates and mitochondrion. Results Drug-contained sera reduced prolifer- ation of HSC-T6 cells and induced apoptosis of MSC-T6 cells compared with control group（ P 〈 0.05 ）. The expressions of Bax in mitochondrion and Caspase-3 in cytoplasm upregulated when cells HSC-T6 were treated with drug-contained sera. Conclusion Caoxianyigan capsule could reduce proliferation of HSC-T6 cells and induce HSC-T6 cells apoptosis, the regulation mecha- nism may be that it change the expression of Bax.

关 键 词：草仙乙肝胶囊 肝星状细胞 线粒体 BAX 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]