Expression of neurocan mRNA and ultrastructure of brain tissue after cerebral ischemia and reperfusion in stroke-prone renovascular hypertensive rats treated by electroacupuncture  

作　　者：Feng Tan Saiying Wan Haike Wu Qiwen HUO Jinliang Wang Wenlin Chen Meifeng Fang Xiaolin Liu Xuewen Wang Jingbo Sun 

机构地区：[1]Department of Neurology, Foshan Hospital of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine, Foshan 528000, Guangdong Province, China [2]Central Laboratory, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510120, Guangdong Province, China

出　　处：《Neural Regeneration Research》2011年第36期2834-2838,共5页中国神经再生研究（英文版）

基　　金：Research Projects of Science and Technology Bureau of Foshan City, No. 04080131;the Administration Bureau of Traditional Chinese Medicine of Guangdong Province, No. 1050006;the Natural Science Foundation of Guangdong Province, No. 8152800007000001

摘　　要：We established a stroke-prone renovascular hypertensive rat model by bilateral constriction of the renal artery with sliver loop clips. After ten weeks, middle cerebral artery occlusion was induced for 2 hours. The rats then received electro-acupuncture at Baihui （DU 20） and Dazhui （DU 14） after onset of ischemia for 30 days. In situ hybridization study showed that electroacupuncture significantly reduced the number of neurocan mRNA-positive cells in the ischemic penumbra and hippocampal tissues of rats. Electron microscopy results demonstrated that the structure of neurons and blood vessels in the ischemic tissues were restored with electroacupuncture. Overall, these data suggest that electroacupuncture may protect neurons against ischemic reperfusion injury in stroke-prone renovascular hypertensive rats, which may be regulated by downregulation of expression of nerve inhibitory factor neurocan mRNA.We established a stroke-prone renovascular hypertensive rat model by bilateral constriction of the renal artery with sliver loop clips. After ten weeks, middle cerebral artery occlusion was induced for 2 hours. The rats then received electro-acupuncture at Baihui （DU 20） and Dazhui （DU 14） after onset of ischemia for 30 days. In situ hybridization study showed that electroacupuncture significantly reduced the number of neurocan mRNA-positive cells in the ischemic penumbra and hippocampal tissues of rats. Electron microscopy results demonstrated that the structure of neurons and blood vessels in the ischemic tissues were restored with electroacupuncture. Overall, these data suggest that electroacupuncture may protect neurons against ischemic reperfusion injury in stroke-prone renovascular hypertensive rats, which may be regulated by downregulation of expression of nerve inhibitory factor neurocan mRNA.

关 键 词：ELECTROACUPUNCTURE cerebral ischemia and reperfusion stroke-prone renovascular hypertensive neurocan mRNA cellular ultrastructure 

分 类 号：Q95-33[生物学—动物学] S858.26[农业科学—临床兽医学]