蒺藜中甾体皂苷对新生隐球菌生物膜形成的抑制作用  

Inhibitory effects of Tribulus terrestris steroid saponin TTS-12 on building biofilm of Cryptococcus neoformans

作　　者：李秀丽[1] 田媛[2] 史玉玲[1] 顾俊瑛[1] 刘至昱[1] 李晓建[1] 高飞[1] 

机构地区：[1]同济大学附属上海第十人民医院皮肤科,上海200072 [2]第二军医大学药学院生化药学教研室,上海200433

出　　处：《中国真菌学杂志》2011年第6期341-343,共3页Chinese Journal of Mycology

摘　　要：目的观察蒺藜甾体皂苷类化合物TTS-12对新生隐球菌生物膜形成的影响,探讨其可能的作用机制。方法光镜观察TTS-12对新生隐球菌生物膜生长形态的影响;MTT法观察TTS-12对新生隐球菌生物膜形成的影响;实时定量RT-PCR观察不同浓度TTS-12对新生隐球菌细胞生物膜关键基因PMT4表达的影响。结果经TTS-12处理的新生隐球菌生物膜结构更疏松,TTS-12可剂量依赖性地降低新生隐球菌生物膜生长动力学指标及PMT4基因表达水平(P<0.01)。结论 TTS-12可抑制新生隐球菌生物膜的形成。通过降低新生隐球菌PMT4基因表达可能是其抑制新生隐球菌生物膜的形成作用机制之一。Objective To investigate the influence of TTS-12,a steroid saponin from Tribulus terrestris,on building biofilm of Cryptococcus neoformans,and to explore the possible mechanism.Methods The inhibitory effects of biofilm formation of Cryptococcus neoformans by TTS-12 was observed by microscopy and assessed by MTT method.The expression of PMT4 mRNA was measured by real-time RT-PCR with different concentrations of TTS-12.Results Compared with control group,TTS-12 treatment showed inhibitory effects on the formation process,making the biofilm more loosened.The growth kinetics and PMT4 mRNA level in TTS-12-treated group were dose-dependently lower than those in the control group（P0.01）.Conclusions TTS-12 may has inhibitory effect on formation of Cryptococcus biofilm by PMT4 gene.

关 键 词：蒺藜 新生隐球菌 生物膜 TTS-12 PMT4 

分 类 号：R379.5[医药卫生—病原生物学]