不同基底静态加载方式对兔肌成纤维细胞(前体)表达α-SMA的影响  

Effect of different substrate static loading on α-SMA expression of rabbit myofibroblasts(protomyofibroblasts)

作　　者：黄岂平[1] 陈国宝[1] 方咸平[1] 

机构地区：[1]重庆大学生物工程学院,重庆400030

出　　处：《医用生物力学》2011年第6期560-565,579,共7页Journal of Medical Biomechanics

基　　金：重庆市自然科学基金计划项目(CSTC;2008BB5057);高等学校学科创新引智计划资助(B06023)

摘　　要：目的通过对不同分化阶段的兔肌成纤维细胞及前体施加不同的基底应变刺激,研究此过程中细胞表达α-SMA的时间剂量关系。方法选取异体蛋白植入法获得5 d和7 d的肌成纤维细胞(前体),培养于弹性膜上;利用基底静态拉伸对其分别施以应变从0%～3%和3%～6%的分段载荷及0%～6%的阶跃式载荷。结果 3 d阶段的肌成纤维细胞(前体)没有表达α-SMA;5～7 d阶段可以监测到α-SMA的表达;在10～15 d阶段则表达显著增强(P<0.01)。两种加载方式下,α-SMA的表达量均显著增加;分段加载作用下SMA的表达量更高。结论肌成纤维细胞(前体)α-SMA受基底静态拉伸后表达增加,且对不同的加载方式能做出不同的响应,提示在肌成纤维细胞的分化和愈伤反应过程中,力学因素扮演着十分重要的角色。Objective To exert different substrate strain stimulation on rabbit myofibroblasts （MFs） （proto-） at different differentiation stages so as to study the variation of a-SMA expression. Methods By implanting heterol- ogous protein into rabbits＇ peritoneal cavity, 5 d and 7 d MFs （proto-） were obtained and cultured on the elastic substrates. Strain of 0%-3% and 3%-6% as well as strain of 0%-6% were applied by substrate static stretch. Results a-SMA expression was not found in 3 d MFs （proto-）, while a-SMA expression could be detected in 5- 7 d MFs （ proto-）, and a-SMA expression was significantly enhanced in 10-]5 d MFs （proto-） （ P 〈 O. O1 ）. Under the two loading modes, a-SMA expression was both significantly improved, and higher a-SMA expression was presented under sectioned loading. Conclusions ^-SMA expression of MFs（proto-） is increased after the sub- strate static stretch in response to different loading modes, which suggests that mechanical factors can play an important role in the process of MFs differentiation and wound-healing response.

关 键 词：肌成纤维细胞 基底 载荷 应变 蛋白表达 分化 

分 类 号：R318.01[医药卫生—生物医学工程]