胶原/羟基磷灰石一体化支架修复关节软骨缺损的机制研究  

Repair of Rabbits Full-thickness Articular Cartilage Defects with Collagen/Hydroxyapatite Scaffold

作　　者：刘兴漠[1] 项禹诚[1] 潘滔[1] 孙青[1] 王德春[1] 王迎军[2] 吴刚[2] 

机构地区：[1]中山大学附属第六医院骨科,广东广州市510655 [2]华南理工大学材料科学与工程学院,广东广州市510641

出　　处：《中国康复理论与实践》2012年第1期37-40,共4页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家自然科学基金(U0834003);广东省科技厅科技计划(2010B031100025);广东省医学科研基金(A2010204)

摘　　要：目的观察新型胶原/羟基磷灰石一体化修复支架材料修复创伤性软骨缺损的生物机制及可行性。方法 45只新西兰兔随机分为3组,实验组和对照组制作全层关节软骨缺损动物模型并在实验组植入新型生物支架材料,正常组不予处理。术后1、2、4周分别处死动物,Wakitani法对修复组织进行组织形态学评分,RT-PCR测定关节滑膜中基质金属蛋白酶(MMPs)-2、3、9和13 mRNA含量及纯度。结果术后2周实验组修复组织主要为透明软骨,对照组无明显修复组织,Wakitani评分两组间有非常显著性差异(P<0.01)。RT-PCR显示实验组MMP-2、3和9的活化降低(P<0.05),而MMP-13在修复过程中持续表达(P>0.05)。结论新型生物支架材料通过抑制MMPs表达水平来减轻炎症反应和保护软骨,在动物体内可促进关节软骨缺损的修复。Objective To investigate the biological mechanisms and feasibility of collagen/hydroxyapatite scaffolds for repairing articular cartilage and subchondral bone defects.Methods 45 New Zealand white rabbits were included.Full-thickness articular cartilage defect models were established in experiment and control groups and the biomaterial scaffolds were implanted in the experiment group.The animals were sacrificed in the 4th,6th and 8th week after the operation respectively.Histological scores were performed by semi-quantity cartilage scoring system and the expressions of matrix metalloproteinases（MMPs）-2,3,9 and 13 mRNA in the synovialis were detected with RT-PCR.Results The repaired tissues were totally hyaline cartilages in the experiment group 2 weeks after operation and there were no repaired tissues in the control group.There were significant differences between these 2 groups in Wakitani scores（P〈0.01）.The activation and expression of MMP-2,3,9 decreased in the experiment group（P〈0.05）,but the activation of MMP-13 sustained and the expression of MMP-13 could be detected during the observed period（P〈0.05）.Conclusion The new biological scaffold can alleviate the inflammatory and protect the cartilages by inhibiting the expression of MMPS,which promote the reconstruction of articular cartilage in rabbits.

关 键 词：软骨缺损 生物支架 基质金属蛋白酶 兔 

分 类 号：R684[医药卫生—骨科学]