胰腺癌裸鼠原位模型3．0TMRI表现  

3. 0 T magnetic resonance imaging of pancreatic cancer in an orthotopic nude murine model

作　　者：刘珍娟[1] 王中秋[2] 杨志坚 安晓静[3] 李林[2] 童明敏[2] 

机构地区：[1]江苏省中西医结合医院放射科,210028 [2]南京军区南京总医院放射科210028 [3]南京军区南京总医院病理科210028 [4]南京源端生物科技有限公司210028

出　　处：《中华医学杂志》2011年第47期3354-3357,共4页National Medical Journal of China

基　　金：国家自然科学基金（30870689,81071175）;江苏省自然科学基金（BK2008325）;南京军区医药卫生科研基金（08MA087）

摘　　要：目的探讨人胰腺癌裸鼠原位模型种植方法，研究其MRI表现及病理基础，为活体研究胰腺癌奠定基础。方法采用人胰腺癌BXPC-3细胞对裸鼠进行原位种植，并使用3．0TMRI成像，包括T，WI、T2WI及增强扫描，结合病理，分析人胰腺癌裸鼠原位模型的MRI表现。结果18只裸鼠有15只接种成功，接种成功率为83．3％（15／18）。病理学检查符合胰腺高分化实体癌。对15只裸鼠行磁共振成像，肿瘤MRI表现为10只T1WI呈均匀稍低信号（66．7％），5只（33．3％）信号不均，以稍低信号为主；T2WI呈均匀（26．7％，4／15）或不均匀（73．3％，11／15）稍高信号，增强扫描瘤灶边缘强化较明显，瘤灶轻度强化，坏死部分不强化。结论3．0TMRI能够检测出直径≥2mm的胰腺肿瘤，并能够清晰显示裸鼠原位模型肿瘤的位置、形态、大小及其内部结构，能为胰腺癌进一步活体研究提供可视化平台。Objective To assess the methods of establishing an orthotopic nude murine model of human pancreatic cancer and explore the manifestations of MRI （magnetic resonance imaging） and its pathological fundamentals so as to provide research rationales for human pancreatic cancer. Methods The BXPC-3 cell of human pancreatic cancer was orthotopically planted in nude mice. And the animals were examined by a clinical 3. 0 T magnetic resonance imager. The MRI examinations were analyzed along with their pathological findings. Results Among 18 mice, 15 of them grew tumors with a success ratio of 83.3%. The pathological findings conformed to those of high differentiation pancreatic parenchyma cancer. Comparing with the neighbor muscles, the tumors were homogeneous （66. 7%, 10/15 ） or heterogeneous （ 33.3 % , 5/15 ） of hypointense on Tl-weighted images while homogeneous （26. 7 % , 4/15 ） or heterogeneous （ 73.3% , 11/15 ） hyperintense on T2-weighted images with heterogeneous enhancement. The border became obviously enhanced and there was mild central enhancement while the necrotic part showed no enhancement. Conclusion 3.0T MRI can detect pancreatic neoplasms ≥2 mm and visualize clearly their locations, shapes, dimensions and internal structures in an orthotopic nude murine model. Thus it provides a visible framework for further studies of human pancreatic cancer.

关 键 词：胰腺肿瘤 磁共振成像 小鼠 裸 模型 动物 

分 类 号：R735.9[医药卫生—肿瘤]