汉族人群肝郁气滞及湿热内蕴型痤疮TLR2 Arg753Gln基因多态性分析  

Analysis of the Toll-like Receptor 2 Arg753Gln Gene Polymorphisms of Acne Patients of Gan-depression Induced Qi Stagnation Syndrome and Damp-heat in the Interior Syndrome

作　　者：田黎明[1] 王玮蓁[1] 杨婷[2] 谢红付[3] 金曌[1] 胡志敏[1] 程杨[1] 胡耀华[4] 彭圆[5] 

机构地区：[1]华中科技大学附属武汉市第一医院皮肤科,武汉430022 [2]昆明医学院第一附属医院皮肤科,昆明650032 [3]中南大学湘雅医院皮肤科,长沙410008 [4]南华大学南华医院,湖南衡阳421002 [5]湖北中医药大学,武汉430065

出　　处：《中国中西医结合杂志》2012年第1期50-53,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：湖南省卫生厅科研基金资助项目(No.2006-238);衡阳市科技局科研基金资助项目(No.2008KS003;2010kj04)

摘　　要：目的探讨汉族人群肝郁气滞及湿热内蕴型痤疮TLR2 Arg753Gln基因多态性,为其发病遗传学研究奠定基础。方法采用PCR-RFLP技术检测75例湿热内蕴型痤疮、87例肝郁气滞型痤疮以及70名健康对照组的TLR2 Arg753Gln基因型分布以及等位基因的频率。结果 Arg/Gln+Gln/Gln基因型于肝郁气滞型为26.44%(23/87)、湿热内蕴型为41.33%(31/75)、健康对照组为12.86%(9/70),各组间比较差异均有统计学意义(P<0.05)。结论 TLR2 Arg753Gln基因多态性中等位基因753Gln的存在,增加了汉族人群肝郁气滞及湿热内蕴型痤疮的发病风险。Objective To study the toll-like receptor 2 （TLR2） Arg753GIn gene polymorphisms of acne patients of Gan-depression induced qi stagnation syndrome and damp-heat in the interior syndrome, thus laying the foundation for genetics studies on its occurrence. Methods The distribution and the frequency of allelic genes were studied in 75 acne patients of damp-heat in the interior syndrome, and 87 acne patients with Gan-depression induced qi stagnation syndrome, as well as 70 healthy subjects （as the normal control group） using polymerase chain reactionrestriction fragment length polymorphism （PCR-RFLP） technique. Results There was signifi- cant difference in the frequency of the genotype Arg/GIn ＋ Gin/Gin in the TLR2 Arg753GIn genetic polymorphisms [26.44% （23/87） in Gan-depression induced qi stagnation syndrome, 41.33% （31/75） in damp-heat in the in- terior syndrome, and 12.86% （9/70） in the normal control group ] （ P 〈 0.05）. Conclusion The existence of 753Gin allele in the Arg753GIn of TLR2 increased the onset risk of acne patients of Gan-depression induced qi stagnation syndrome and damp-heat in the interior syndrome.

关 键 词：痤疮 肝郁气滞型 湿热内蕴型 TOLL样受体2 基因多态性 

分 类 号：R275.9[医药卫生—中西医结合]