蜕皮甾酮对H_2O_2诱导氧化损伤的人晶状体上皮细胞核因子κBp65表达的影响  

Effects of Ecdysterone on the Expression of NF-κB p65 in H_2O_2 Induced Oxidative Damage of Human Lens Epithelial Cells

作　　者：冯春燕[1] 黄秀榕[2] 祁明信[1] 唐宋文[2] 郭娜[1] 胡艳红[1] 陈胜[1] 柯发杰[1] 

机构地区：[1]福建中医药大学附属第二人民医院眼科,福州350003 [2]福建中医药大学病理生理研究中心,福州350003

出　　处：《中国中西医结合杂志》2012年第1期76-79,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：福建省卫生厅青年科研课题(No.2009-1-30);福建省教育厅课题(No.JA10176)

摘　　要：目的探讨具有雌激素活性的牛膝的有效中药单体蜕皮甾酮(ecdysterone,ECR)对过氧化氢(H2O2)诱导氧化损伤的人晶状体上皮细胞(human lens epithelial cell,HLEC)核因子κB(nuclear factor-kap-paB,NF-κB)p65表达的影响。方法实验分5组:正常组、H2O2组、雌二醇(E2)组、ECR组、核因子抑制剂二硫代氨基甲酸吡咯烷(pyrrolidine dithiocarbamate,PDTC)组;采用流式细胞术(FCM)测定各组标本中HLEC的NF-κBp65的表达率。结果正常HLEC能表达一定的NF-κBp65(9.53%),H2O2组NF-κBp65的表达率明显增高(39.87%,P<0.01),PDTC组则明显降低(5.90%,P<0.01),ECR组(13.99%)和E2组(25.18%)介于正常组与H2O2组之间,均低于H2O2组(P<0.01)。结论 H2O2可以诱导HLEC的NF-κB的活化表达,具有雌激素活性的中药单体ECR可以有效抑制NF-κB的活化表达。Objective To study the effects of ecdysterone （ECR） on the expression of nuclear factor （NF）-KB in H2O2induced oxidative damage of human lens epithelial cells （HLECs）. Methods The cultured HLECs were divided into 5 groups, i.e., the control group, the H2O2 group, the β-estradiol （E2） group, the ECR group, and the pyrrolidine dithiocarbamate group （PDTC） group. The expression rate of NF-KB p65 in the HLECs were detected by flow cytometer （FCM）. Results The expression of NF-KB p65 occurred in normal HLECs （9. 53%）. The expression rate of NF-KB 1065 in the H202 group obviously increased （39. 87%, P〈0. 01 ）. The expression rate of NF-KB p65 in the PDTC group obviously decreased （5. 90%, P〈0. 01 ）. The expression rates of NF-KB p65 in the ECR group （13.99%） and the E2 group （25.18%） ranged between the control group and the H202 group, but still lower than that of the H202 group （P〈0.01）. Conclusions The activation of NF-KB in the HLECs could be induced by H2O2. ECR with the estrogenic activity could effectively inhibit the activation of NF-KB.

关 键 词：蜕皮甾酮 核因子ΚB 人晶状体上皮细胞 氧化损伤 

分 类 号：R285.5[医药卫生—中药学]