hS100A6抑制卵巢癌细胞HO8910和HO8910 PM增殖、促进凋亡并上调其Wnt/β-catenin活性  

hS100A6 inhibits proliferation,induces apoptosis and up-regulates Wnt/β-catenin activity in ovarian cancer cell lines HO8910 and HO8910 PM

作　　者：邹正渝[1] 陈英华 孙双双[1] 黎玉叶[1] 叶立伟[1] 段亮[1] 武睿[1] 杨霞[3] 罗进勇[1] 周兰[1] 

机构地区：[1]重庆医科大学医学检验系临床检验诊断学教育部重点实验室,重庆400016 [2]重庆市第四人民医院肿瘤科,重庆400015 [3]重庆医科大学附属口腔医院口腔黏膜科,重庆400015

出　　处：《第三军医大学学报》2012年第3期214-218,共5页Journal of Third Military Medical University

基　　金：国家自然科学基金面上项目(30772548)~~

摘　　要：目的研究hS100A6(human S100A6)对人卵巢癌细胞系(低转移系HO8910和高转移系HO8910 PM)增殖、凋亡和Wnt/β-catenin信号途径的影响。方法通过MTT以及台盼蓝染色检测细胞增殖的变化;Hoechst检测细胞凋亡的变化;提取细胞总蛋白及核蛋白Western blot检测hS100A6对β-catenin的影响。结果 MTT及台盼蓝染色结果显示hS100A6抑制HO8910细胞和HO8910 PM细胞的增殖(P<0.05),Hoechst染色结果显示hS100A6同时促进这两系细胞的凋亡(P<0.05);Western blot显示hS100A6增加β-catenin的水平并促进其发生核转位。结论 hS100A6能抑制人卵巢癌细胞系HO8910和HO8910 PM增殖,促进其凋亡,同时激活Wnt/β-catenin信号途径。Objective To investigate the effect of human S100A6（hS100A6） on the proliferation,apoptosis and Wnt/β-catenin signaling pathway of ovarian cancer cell line HO8910 and HO8910 PM.Methods MTT assay and trypan blue exclusion assay,Hoechst staining and Western blotting were used to detect cell proliferation,apoptosis and expression and distribution of β-catenin,respectively.Results ① hS100A6 inhibited proliferation of HO8910 and HO8910 PM cells in a dose-and time-dependent manner（P0.05）;② hS100A6 induced apoptosis in HO8910 and HO8910 PM cells（P0.05）;③ Level of β-catenin was increased by hS100A6 in HO8910 and HO8910 PM cells,and β-catenin translocated from cytoplasm to nucleus.Conclusion hS100A6 can inhibit cell proliferation,induce cell apoptosis and up-regulate Wnt/β-catenin activity in ovarian cancer HO8910 and HO8910 PM cells.

关 键 词：S100A6 卵巢癌 Wnt/β-catenin信号途径 

分 类 号：R341[医药卫生—基础医学] R730.23