西洋参皂甙的抗氧化功能及其对小鼠遗传损伤的保护作用  

Protective effect on genetic damage of mice and antioxidation of panax ginsenoside

作　　者：吴华彰[1] 赵云利[2] 费鸿君[1] 李静[1] 刘晓佳[3] 吴守伟[1] 

机构地区：[1]蚌埠医学院生物科学系,安徽蚌埠233030 [2]蚌埠医学院预防医学系,安徽蚌埠233030 [3]蚌埠医学院临床医学系,安徽蚌埠233030

出　　处：《中国生物制品学杂志》2012年第1期61-64,共4页Chinese Journal of Biologicals

基　　金：2011年度安徽高校省级科学研究项目(KJ2011Z252);安徽省高等学校省级教学研究项目(2008jyxm431);蚌埠医学院自然科学项目(BY0751)

摘　　要：目的探讨西洋参皂甙的抗氧化功能及其对环磷酰胺(Cyclophosphamide,CP)所致小鼠遗传损伤的保护作用。方法应用总抗氧化能力(Total Antioxidant Capacity,TAC)和抑制超氧阴离子(O2-)活力试剂盒检测西洋参皂甙的TAC和抑制O2-的活力;水杨酸比色法检测羟自由基(OH-)的清除能力;邻苯三酚自氧化法检测O2-自由基的清除能力。给昆明小鼠每天灌胃不同浓度(10、50、200 mg/kg)的西洋参皂甙,第6天起按25 mg/kg以CP染毒,连续5 d,用试剂盒检测血清谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性及丙二醛(MDA)的水平;常规Giemsa染色观察小鼠骨髓细胞微核的形成并计算微核形成率;构建小鼠遗传损伤模型,常规Giemsa染色观察小鼠精子畸变程度并计算精子畸变率。结果西洋参皂甙在体外模拟系统中的TAC和抑制O2-生成活力较高,分别达80和160 U/ml,对OH-和O2-的清除率达70%～80%,且呈剂量效应关系;西洋参皂甙干预可显著提高CP染毒小鼠血清中GSH-Px和SOD的活性(P<0.01),降低MDA的水平(P<0.05),拮抗CP对精子和骨髓细胞的损伤作用,西洋参皂甙各剂量组小鼠的精子畸变率和微核形成率与模型组相比,均明显下降(P<0.01)。结论西洋参皂甙具有较强的体内外抗氧化活性,对CP所致小鼠遗传损伤具有明显的保护作用,其机制可能与提高机体的抗氧化能力和增强小鼠的抗诱变能力有关。Objective To investigate the antioxidation of panax ginsenoside as well as its protective effect on genetic damage of mice caused by cyclophosphamide （CP）. Methods Panax ginsenoside was determined for total antioxidant capacity （TAC） and activity in inhibiting superoxide anion radicals （O2-） of panax ginsenoside by the corresponding kits, and for clearances of hydroxyl radicals （OH-） and 02- by salicylic acid colorimetry and pyrogallol autoxidation method respectively. Kunming mice were immunized ＂with panax ginsenoside at various dosages （10, 50 and 200 mg/ kg） by garage once a day and, starting from day 6, injected i. p. with CP at a dosage of 25 mg/kg for 5 d. The glutathione peroxidase （GSH-Px） and superoxide dismutase （SOD） activities as well as malondialdehyde （MDA） level in sera of mice were determined by the corresponding kits. The formation of mieronucleus in mouse bone marrow cells by routine Giemsa staining, based on which the micronucleus formation rate was calculated. Mouse model of genetic damage was established, and observed for sperm aberration by routine Giemsa staining, based on which the sperm aberration rate was calculated. Results The TAC and activity in inhibiting the production of 02- of panax ginsenoside in mimic system in vitro were 80 and 160 U/ml respectively, while the clearances of OH- and 02- were 70% - 80%, all of which were dosage-dependent. The intervention by panax ginsenoside increased the GSH-Px and SOD activities （P 〈 O. O1 ） and decreased MDA level in sera of mice challenged with CP significantly （P 〈 O. 05）, and antagonized the damage effect of CP on sperm and bone marrow cells. Both the sperm aberration rate and micronucleus formation rate of mice immunized with panax ginsenoside at various dosages decreased significantly as compared with those unimmunized （P 〈 O. 01）. Conclusion Panax ginsenoside showed high in vitro and in vivo antioxidation activities as well as significantly protective effect on genetic d

关 键 词：西洋参 皂甙类 抗氧化 环磷酰胺 遗传损伤 

分 类 号：R93[医药卫生—生药学]