硫化氢在脓毒症大鼠心肌损伤中的作用  

Effects of hydrogen sulfide on sepsis-induced cardiac dysfunction

作　　者：陈娣[1] 潘昊[1] 李醇文[2] 兰秀彩[1] 刘蓓蓓[1] 杨光田[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院急诊科,武汉430030 [2]重庆医科大学附属第二医院急救部

出　　处：《中华急诊医学杂志》2012年第1期48-53,共6页Chinese Journal of Emergency Medicine

基　　金：国家自然科学基金（81071526）

摘　　要：目的研究硫化氢（H：s）在脓毒症大鼠心肌损伤中的作用。方法雄性sD大鼠60只，月龄3～4个月，体质量180—220g，随机（随机数字法）分为4组：对照组（I组）、脓毒症组（II组）、脓毒症＋NariS（H，s供体）预处理组（Ⅲ组）、脓毒症＋PAG（H：s代谢酶CSE抑制剂）组（Ⅳ组），每组各15只。采用盲肠结扎穿孔法制作脓毒症大鼠模型，观察各组大鼠血流动力学改变，测定心肌组织中H，s的变化，采用RT-PCR方法观察CSEmRNA表达，采用髓过氧化物酶（MPO）测定试剂盒测定各组心肌组织MPO含量，用ELISA方法检’坝盯心肌组织TNF-a、IL-1B的表达，光学显微镜下观察心肌形态学改变。结果与I组相比，Ⅱ组血压下降，心肌组织中H2S、TNF-a、IL-1B、MPO含量出现不同程度增加（P〈0．01或P〈0．05），CSEmRNA表达水平提高（P〈0．05）；与Ⅱ组相比，Ⅲ组血压下降更为显著，心肌组织中H：S、TNF-a、IL-1p含量增加，MPO活性增强（P〈0．05），但CSEmRNA表达水平并无显著改变；与Ⅱ组相比，Ⅳ组血压水平接近，心肌组织中H2S、TNF—d、IL-1p含量均降低，MPO活性减弱（P〈0．05），CSEmRNA表达水平下降（P〈0．05）。四组心肌组织形态学损伤由轻到重依次为I、Ⅳ、Ⅱ、Ⅲ。结论脓毒症大鼠心肌组织CSE／H：S体系上调，给予H：s可以升高脓毒症大鼠心肌组织中MPO、TNF．仪、IL-1B水平，加重心肌损伤，给予H，s抑制剂可以减轻心肌损伤。Objective To investigate the effects of hydrogen sulfide on sepsis-induced cardiac dysfunction in rats. Methods Male SD rats were randomly （ random number ） divided into 4 groups： control group （ I group） , sepsis group （ ]I group） , sepsis ＋ Naris group （ HI group） , sepsis ＋ PAG group （IV group）. Cecal ligation and puncture technique was used in H, m, IV group. Hemodynamic was observed, and H2S synthesis , CSE （cystathionine-r-lyase） mRNA, MPO activity and the level of TNF-a, IL-1Bwere determined. The morphological changes and infiltration of inflammatory cells in myoeardium were also observed. Results Compared with I group, H2 S synthesis, the levels of TNF-a, IL-1B, the activity of MPO increased （P 〈 0. 05 or P 〈 0. 01 ） , and the expression of CSE-mRNA increased, and blood pressure of rats decreased significantly in II group. Compared with group , H2S synthesis, the levels of TNF-a, IL-1B and the activity of MPO increased （ P 〈 0. 05 ）, the expression of CSE mRNA did not change noticeably （ P 〉 0. 05 ） , and blood pressure of rats decreased more significantly in III group. Compared with II group , H2S level decreased, the levels of TNF-a, IL-lB and the activity of MPO decreasedsignificantly, the expression of CSE mRNA decreased and blood pressure of rats decreased in VI group （ P 〈 O. 05）. Histopathological changes of myocardium were aggravated in the following severity orderm. Conclusions CSE/H2S system of the myocardium was upregulated in sepsis rats. Hydrogen sulfide could raise the levels of MPO, TNF-a, IL-B, aggravating myocardial injury. Contrarily, the inhibitor of H2 S could counteract it.

关 键 词：硫化氢 脓毒症 炎症 心肌 

分 类 号：R459.7[医药卫生—急诊医学]