白藜芦醇对高脂饮食大鼠骨骼肌不同线粒体亚群氧化、抗氧化水平和胰岛素敏感性的影响  

Effect of resveratrol on the oxidative stress and antioxidant levels of different skeletal muscle mitochondrial subpopulations and insulin sensitivity in rats fed with high.fat diet

作　　者：陈璐璐[1] 张好好[1] 郑涓[1] 胡祥[1] 孔雯[1] 胡帝[1] 王素星[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院内分泌科,武汉430022

出　　处：《中华内分泌代谢杂志》2012年第1期58-62,共5页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金资助项目（30771035）

摘　　要：目的 观察白藜芦醇对高脂饮食大鼠骨骼肌不同线粒体亚群氧化、抗氧化水平及胰岛素敏感性的影响。方法8周龄雄性sD大鼠分为普通饮食组（NC组）、高脂饮食组（HF组）及白藜芦醇干预高脂饮食组（HFR组）；干预8周后检测各组大鼠骨骼肌肌膜下（SS）及肌纤维间（IMF）线粒体氧化应激及抗氧化水平，并观察各组大鼠整体及骨骼肌胰岛素敏感性的变化。结果与NC组相比，HF组大鼠胰岛素敏感性明显下降（P〈0．05），SS及IMF线粒体活性氧簇（ROS）和丙二醛的水平明显增加，ss线粒体抗氧化酶活性均下降，而IMF线粒体抗氧化酶活性均增高（均P〈0．05）。与HF组相比，HFR组胰岛素敏感性明显改善，SS及IMF线粒体的抗氧化酶活性均明显增高，ROS和丙二醛的含量明显下降到NC组水平（均P〈0．05）。结论白藜芦醇对高脂饮食大鼠骨骼肌不同线粒体亚群氧化应激水平均有明显的改善作用，并且显著增加其胰岛素敏感性。Objective To observe the effect of resveratrol on the oxidative stress and antioxidant levels of different mitochondrial subpopulations in the skeletal muscle and insulin sensitivity of rats fed with high-fat diet. Methods Male SD rats, aged 8 weeks, were divided into normal chow （ NC ） group, high-fat diet （HF） group, high-fat diet plus resveratrol （HFR） group. After intervention for 8 weeks, the impacts of resveratrol on oxidative stress levels and antioxidant enzymes activities in subsarcolemmal （SS） and intermyofibrillar （IMF） mitochondria from skeletal muscle as well as general and skeletal mascle insulin sensitivity were assessed. Results Compared with NC group, insulin sensitivity was significantly reduced while reactive oxygen species （ROS） and malondialdehyde （MDA） levels in SS and IMF mitochondria increased in HF group （ all P 〈 O. 01 ）. In addition, antioxidant enzyme activities were significantly decreased in SS mitochondrial and increased in IMF mitochondrial （both P 〈 0.05 ）. Compared with HF group, the insulin sensitivity in HFR group was significantly improved. Moreover, the activities of antioxidant enzymes in SS and IMF mitochondrial were increased, and the oxidative stress levels returned to normal （ P 〈 O. 05 ）. Conclusion Resveratrol notably improves the oxidative stress of different skeletal muscle mitochondrial subpopulations and insulin resistance in rats fed with high-fat diet.

关 键 词：白藜芦醇 肌膜下线粒体 肌纤维间线粒体 胰岛素抵抗 氧化应激 

分 类 号：R285.5[医药卫生—中药学]