胰岛素样生长因子-1对成纤维细胞向成骨细胞转化的影响  

The effect of insulin-like growth factor-I on the osteogenic phenotype of fibroblasts

作　　者：孙海钰[1] 张彬[1] 管涛[2] 刘亮[1] 王东[1] 

机构地区：[1]山西医科大学第二医院骨科,太原030001 [2]山西临汾市人民医院骨科

出　　处：《中华创伤骨科杂志》2012年第1期49-52,共4页Chinese Journal of Orthopaedic Trauma

基　　金：山瞳省归国留学人员科研基金（30772189）

摘　　要：目的探泔胰岛素样生长因子-1对成纤维细胞（Fb）向成骨细胞转化的影响。方法F11来源于成年新州兰大白兔，通过分离、纯化、培养而得到。实验分为3组：空白对照组、成骨诱导组和实验组。空白对照纰：常规培养液培养，不加入仃何诱导剂及干预因子；成骨诱导组：成骨诱导液为常规培养液中加入浓度为l×10^-8mol／L地塞米松、50mg／L维生素C、10mmol／Lβ-甘油磷酸钠；实验组：成骨诱导液中加入终浓度为50ng／mL的IGF-1。采用四甲基偶氮唑蓝（MTF）比色法检测各组细胞增殖情况，检测各组细胞碱性磷酸酶（ALP）活性，测定各组细胞培养后6d后的骨钙素含量，采用钙钴染色评价培养后2周的成骨能力。结果实验组细胞增殖较其他两组旺盛，差异有统计学意义（P〈0．05），而空白对照组和成骨诱导组差异无统计学意义（P〉0．05）。ALP活性和骨钙素测定：实验组ALP表达明显高于其他两组，差异有统计学意义（P〈0．05）；成骨诱导组ALP表达高于空门对照组，差异有统计意义（P〈0．05）。实验组钙化结节数量明显较其他两组多，成骨诱导组次之，空白对照组未见钙化结节。结论胰岛素样生长因子-1口丁以促进Fb在成骨过徉中的增殖以及增加其成骨能力。Objective To investigate the effect of insulin-like growth faetor-I （1GF-I） on tile os- teogenic phenotype of fihrohlasts. Methods Fihroblasts （Fbs） were derived fi＇om an aduh New Zealand white rabbit through isolation, purification and cultivation. The experiment was conducted in 3 groups. In the control group, Fhs were euhured in conventional medium without any interw^ntion factors. In the osteogenic induction group, Ihe osteogenic induction medium was composed of conventional medium plus dexamethasone al concentration of 1 x 10s mol/L plus vitamin C at 50 mg/L plus sodium glycerophosphate-[3 at 10 mmol/L. In the experimental group, Fbs were euhured in osteogenic induction medimn plus IGF-I at the final con- centration of 50 ng/mL. Proliferation of Fbs in each group was detected by methyl thiazolyl tetrazolium （MTT） colorimelrie assay. Alkaline phosphatase （ALP） activily was detected, osteoealcin 6 days after cuhure was determined, and lhe ability of osteogenie differenliation 2 weeks after cuhure was evaluated by caleium-cobah staining. Results MTT showed that the cells grew significantly faster in the experimental group than in the other 2 groups（ P 〈 0.05）, but there was no significant difference between the control group and the osteogenic induelion group in this respect （ P 〉 0.05） . The ALP expression in the experimental group was insignificantly higher lhan in the osteogenic induction group（ P 〈 O. 05）, but significantly higher than in the control group （ P 〈 O. 05）. In osteoealcin secretory aetivity, the experimental group was significantly superior to the other 2 groups and the osteogenie induction group was significantly superior to the control group （ P 〈 O. 05）. The experimental group had significantly more calcified nodules than the uther 2 groups and there were few calcified nodules in the control group 2 weeks after cuhure. Conclusion IGF-1 can advance pro- liferation and osleogenie phenolype expression of the Fb stinmlated.

关 键 词：胰岛素样生长因子-1 成纤维细胞 细胞增殖 

分 类 号：R681[医药卫生—骨科学]