兔卵母细胞体外成熟培养影响因素的研究  

Study on the influencing sectors of in vitro maturation and fertilization of rabbit oocytes

作　　者：石金月[1] 杨金姬[1] 向舒[1] 张顺[1] 苏丽琼[1] 蒋建荣[1] 石德顺[1] 陆凤花[1] 

机构地区：[1]广西大学动物繁殖研究所/广西亚热带生物资源保护利用重点实验室,南宁530004

出　　处：《黑龙江畜牧兽医》2011年第12期1-3,6,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金："863"国家高技术研究发展计划项目(2008AA10Z160)

摘　　要：为了研究兔卵巢保存温度、运输时间及季节对兔卵母细胞体外成熟培养效果的影响,试验把从屠宰场获取的兔卵巢分别保存在25～30℃、31～35℃、36～40℃的生理盐水中运回实验室,将采集到的卵母细胞体外成熟22～24 h,比较兔卵巢分别在<3 h、≥3～≤4 h、>4 h 3个不同时间段运回实验室对卵母细胞体外成熟的影响。结果表明:卵巢保存在25～30℃生理盐水时,卵母细胞体外成熟率为61.09%,显著高于另两者(26.30%、15.79%,P<0.05)。卵巢运输时间>4 h时,卵母细胞的成熟率为50.23%,显著低于另两者(64.24%5、9.64%,P<0.05)。夏季从屠宰场收集兔卵巢得到卵母细胞体外成熟率为32.65%,显著低于春季、秋季和冬季(64.26%、58.37%6、4.86%,P<0.05),但春季、秋季和冬季之间差异不显著(P>0.05)。说明在春季、秋季和冬季收集兔卵巢,放在25～30℃生理盐水中保存,在4 h内送回实验室,有利于卵母细胞的成熟培养。To study the effects of in vitro maturation（IVM） of rabbit oocytes,rabbit ovaries were collected under different seasons,temperature and storage time.The experiment adopted the rabbit ovaries to be placed in normal saline at various temperature of 25-30 ℃,31-35 ℃,36-40 ℃ respectively.The ovaries would be transported to a laboratory as quickly as possible.And then the rabbit oocytes collected were cultured in maturation system for 22-24 hours.The rabbit oocytes in vitro maturation were studied during different storage time at 3 h,≥3-≤4 h,4 h affected.The different seasons were compared to reveal the effect on rabbit oocytes in vitro maturation with the same ovaries storage temperature and transport time.The results showed that the proportion of maturation in temperature 25-30 ℃ group was 61.09%,significantly higher than 31-35 ℃ and 36-40 ℃ groups（26.30% 15.79%,P0.05）.The maturation rate of 4 h group of transported time was 50.23%,which was significantly lower than the 3 h and ≥3-≤4 h groups（64.24%,59.64%,P0.05）.The summer group had obtained a percentage rate of matured oocytes 32.65%,significantly lower than that of spring,autumn and winter groups（64.26%,58.37%,64.86%,P0.05）,but there were no significant differences among the three groups.The study demonstration that the effect of collecting and culturing rabbit oocytes in spring,autumn and winter would be better,and the ovaries were stored at a 25-30 ℃ physiological saline and ovaries transported to the lab not exceed four hours would get a pretty maturation rate.

关 键 词：兔 卵母细胞 体外成熟 保存温度 运输时间 季节 

分 类 号：S829.1[农业科学—畜牧学]