缺氧缺血新生鼠脑损伤后NGF对Nogo-A蛋白表达的影响  

Influence of NGF on neural stem cell Nogo-A expression after cerebral hypoxia ischemic in newborn rats

作　　者：宋文秀[1] 范勇波[1] 吴晓媚[2] 

机构地区：[1]遵义医学院第五附属医院儿科,广东珠海519100 [2]遵义医学院第五附属医院病理科,广东珠海519100

出　　处：《中国优生与遗传杂志》2011年第12期28-29,F0003,共3页Chinese Journal of Birth Health & Heredity

基　　金：珠海市科技局项目

摘　　要：目的研究外源性神经生长因子(NGF)对新生鼠缺氧缺血性脑损伤(HIBD)后Nogo-A表达的影响。方法 72只7d龄SD大鼠随机分为NGF治疗组、对照组和假手术组,应用免疫组化的方法观察NGF对脑组织Nogo-A表达的影响。结果脑组织Nogo-A的表达在对照组和NGF治疗3 d开始增高,21 d达到最高峰,对照组增高幅度最大,其矫正吸光度值与假手术组和NGF治疗组比较有显著性差异(P<0.05)。结论 NGF对HIBD具有神经修复作用,可能是通过减少Nogo-A的表达而实现的。Objective： To study the effects of exogenous nerve growth factor （NGF） on expression of Nogo -A in the neonatal rat brain tissue after hypoxia ischemic brain damage （HIBD）. Methods ： 72 neonatal SD rats were divided randomly into NGF group, controlled group and sham - operated group, Immunohistochemistry method was used to measure the expressions of Nogo - A after in the neonatal rat brain tissue after hypoxia ischemic brain damage. Results ： The expression of Nogo - A in the neonatal rat brain tissue in NGF group and controlled group increased at 3 d after hypoxia ischemic brain damage and reached the highest level at 21 d; the corrected optical density （COD） value of controlled group was significantly higher than that of the sham - operation group and NGF group （P 〈 0. 05 ）. Conclusion ： The neuro - protective effect of NGF on the newborn rats with HIBD is related to the reduced expression of Nogo - A.

关 键 词：NGF NOGO-A 缺氧缺血性脑损伤 (HIBD) 

分 类 号：R722.1[医药卫生—儿科]