妊娠期高血压疾病患者胎盘组织中抵抗素的表达  

The expression and significance of resistin in the placenta of hypertensive disorder complicating pregnancy

作　　者：孙玉红[1] 陈萱[1] 程国斌 吕楠[1] 林楠[3] 

机构地区：[1]哈尔滨医科大学附属第二医院,黑龙江150086 [2]黑龙江省人口计划生育信息中心 [3]哈尔滨医科大学附属第四医院

出　　处：《中国优生与遗传杂志》2011年第12期61-63,共3页Chinese Journal of Birth Health & Heredity

基　　金：胎盘HO系统与先兆子痫发病机理的研究项目编号:2005AA9CS116-11

摘　　要：目的检测抵抗素(Resistin)在妊娠期高血压疾病患者胎盘组织中的表达,探讨抵抗素在妊娠期高血压疾病发病机制中的作用。方法选取妊娠期高血压疾病患者40例,包括妊娠期高血压患者20例,子痫前期患者20例,选取无高血压、糖尿病等并发症的孕妇20例作为对照组。并记录每个患者新生儿出生体重。应用免疫组化SP两步法检测抵抗素在其胎盘组织中的表达情况,对免疫组化切片采用Tanaka等级评分法进行评分。应用studengt t检验,分别对子痫前期组与对照组,妊娠期高血压组与对照组免疫组化评分结果进行比较;并采用双变量相关法,估计胎盘抵抗素的表达与新生儿出生体重的相关性,以P<0.05为差异有显著性。结果本研究发现抵抗素表达于胎盘绒毛滋养细胞的细胞浆中;对免疫组化切片应用Tanaka等级评分法进行评分,经studengt t检验发现子痫前期组抵抗素在胎盘组织中的表达低于对照组,P<0.05,差异有显著性;妊娠期高血压组与对照组差异无显著性,P>0.05。抵抗素在胎盘组织中的表达与新生儿出生体重正相关(r=0.883,P<0.05)。结论抵抗素可能参与了子痫前期的发病机制,推测其表达下降是由胎盘功能减退引起的,这可能是疾病发展过程中的一个结果而不是原因;抵抗素可能参与了子痫前期胎儿生长受限的发生,其具体机制将有待于进一步研究。Objective： To investigate the nosogenesis of hypertensive disorder complicating pregnancy by detecting the expression of resistin in the placenta. Methods ： We selected 20 cases of hypertensive disorder complicating pregnancy and 10 cases of normal pregnancy. The former contained 10 cases of gestational hypertension, whose pressure were ≥ 140/90mmHg and urine protein were negative; and another 10 cases were pre - eclampsia, whose pressure fluctuated in the range of 140 - 180/90 - 125mmHg and urine protein were （ ＋ - ＋ ＋ ＋ ＋ ）. The expression of resistin was detected by streptavidin - peroxidase immunohistochemistry with appropriate antibodies. Results ： Resistin was positively expressed in the plasm of placenta villus trophoblastic cells. The expression of resistin that in pre - eclampsia were markedly lower than those of control, P 〈 0. 05 ; no significant differences between gestational hyperten- sion and the control, P 〉 0.05. The expression of resistin and the birth weight was strongly correlated （r = 0. 883, P 〈 0. 05）. Conclusion： Resistin meybe play a role in the nosogenesis of hypertensive disorder complicating pregnancy. The expression of resistin in the placenta declined maybe caused by the depressed placental function when pre - eclampsia occurred. It can be inferred that resistin is not the cause but the result in the nosogenesis of pre - eclampsia. Resistin may be taken part in the occurance of fetal growth restriction in the pre - eclampsia.

关 键 词：妊娠期高血压疾病 胎盘 抵抗素 子痫前期 免疫组化 

分 类 号：R714.246[医药卫生—妇产科学]