ELISA与免疫传感器在大肠杆菌内毒素检测中的应用  

Application of ELLSA and immunosensor in detection of Escherichia coli endotoxin

作　　者：郭寅生[1] 郝春慧[1] 刘红艳[1] 黄正[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院教育部环境与健康重点实验室,武汉430030

出　　处：《中国卫生检验杂志》2011年第12期2884-2887,共4页Chinese Journal of Health Laboratory Technology

基　　金：国家自然科学基金项目(30771774)

摘　　要：目的:研究两种基于免疫反应和电化学的简便、灵敏、特异检测内毒素的新方法。方法:应用ELISA建立PMB-ET-ELISA法和免疫传感器法对大肠杆菌内毒素进行分析,并对两种方法检测范围和最低检测限进行分析和比较。结果:ELISA方法检测精制内毒素范围为1μg/ml～1 mg/ml,检测大肠杆菌O157:H7范围为105 CFU/ml～109 CFU/ml,相当于内毒素190μg/ml～2918μg/ml。免疫生物传感器法检测精制内毒素范围为1μg/ml～1 mg/ml;检测大肠杆菌O157:H7范围为106 CFU/ml～109 CFU/ml,相当于内毒素288μg/ml～1416μg/ml。结论:建立的ELISA和免疫传感器两种检测内毒素新方法有效、稳定、可用。Objective：To study two electrochemical methods for the detection of endotoxin based on simpleness,sensitivity,and specificity.Methods： Escherichia coli endotoxin were analyzed by PMB-ET-ELISA and immunosensor.Moreover,the detection range and the detection limit of the two methods were analyzed and compared.Results： With ELLSA（enzyme-linked immunosorbent assay）,the detection range of refined endotoxin were 1 μg/ml^1 mg/ml,E.coli O157： H7 detection range were 105 CFU/ml^109 CFU/ml,equivalent to endotoxin 190 μg/ml^2918 μg/ml.While with immunosensors,the detection range of refined endotoxin were 1 μg/ml^1 mg/ml;detection range of E coli O157：H7 were 106 CFU/ml^109 CFU/ml,equivalent to endotoxin 288 μg/ml^1416 μg/ml.Conclusion： The ELISA and immunsensor in endotoxin detection are effective,stable and feasible.

关 键 词：ELISA 免疫传感器 大肠杆菌内毒素 

分 类 号：R446.5[医药卫生—诊断学]