血浆凝固酶试验直接鉴定血培养阳性金黄色葡萄球菌  

Direct identification of Staphylococcus aureus in blood cultures by coagulase test

作　　者：黄黎俐[1] 杨青[2] 骆骥才[2] 滕敏[2] 

机构地区：[1]浙江省丽水市人民医院,浙江丽水323000 [2]浙江大学医学院附属第一医院,杭州310003

出　　处：《中国卫生检验杂志》2011年第12期2888-2889,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:利用血浆凝固酶试验快速鉴定血培养阳性瓶中的金黄色葡萄球菌。方法:血培养仪阳性报警后,立即采集血培养阳性标本进行革兰染色,涂片结果判断为葡萄球菌的标本进行血浆凝固酶试验,同时按常规方法进行分离培养,挑取纯培养菌落用VITEK 2 Compact全自动细菌鉴定仪进行菌种鉴定。结果:2010年3月-2010年6月份共收集100份血培养阳性涂片疑似为葡萄球菌标本,进行血浆凝固酶试验,其中血浆凝固酶阳性的有23份,菌种经VITEK 2Compact仪器鉴定均为金黄色葡萄球菌,血浆凝固酶阴性的有77份,菌种经鉴定均为血浆凝固酶阴性葡萄球菌。血浆凝固酶试验最快1h即可出现阳性结果。结论:直接对血培养瓶原液进行血浆凝固酶试验可用于金黄色葡萄球菌的鉴定。Objective：By utilizing the plasma-coagulase test to quickly identify the Staphylococcus aureus in blood culture positive bottles.Methods： When the blood culture positive alarms,the positive samples were collected to take the Garm stain immediately.Then samples whose smear results were identified as Staphylococcus were used for plasma coagulase test,at last using the VITEK 2 Compact instruments to identify these pure cultures.Results： From March to June in 2010,there have been 100 smears from positive blood cultures suspected as Staphylococcus to take the plasma coagulase test,including 23 coagulase positive cultures,which were all identified as Staphylococcus by VITEK 2 compact instruments.77 coagulase negative cultures were coagulase negative staphylococci.Plasma coagulase test will be completed quickly in one hour.Conclusion： Directly using the blood cultures to do the plasma-coagulase test can be used for the identification of the Staphylococcus aureus

关 键 词：血浆凝固酶试验 血培养 金黄色葡萄球菌 

分 类 号：R446.5[医药卫生—诊断学]