探讨微阵列酶联免疫法在艾滋病哨点监测工作中的应用  

Study of microarray enzyme-linked immunosorbent assay in detection of pathogens in AIDS workstation

作　　者：张建涛[1] 徐志鑫[1] 郝志波[2] 

机构地区：[1]北京市昌平区疾病预防控制中心,北京102200 [2]北京华大吉比爱生物技术有限公司,北京101300

出　　处：《中国卫生检验杂志》2011年第12期2896-2897,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:探讨微阵列酶联免疫法检测的临床价值。方法:用酶联免疫吸附试验(ELISA)和微阵列酶联免疫法(Ar-ray-ELISA)检测试验组样本的抗-HIV抗体、抗-TP抗体。分别统计两种方法检测阳性、阴性的样本数目,并进行统计学处理。结果:两种方法分别检测400份实验组样本,统计检测结果并经卡方检验证明无差别。检测结果的总符合率均为100%。结论:微阵列酶联免疫法与ELISA法无统计学差异,初步证明可以满足艾滋病哨点监测工作中检测工作的要求。Objective：To investigate the clinical diagnostic value of the microarray enzyme-linked immunosorbent assay（Array-ELISA）.Methods： Total samples were tested by enzyme-linked immunosorbent assay（ELISA） and Array-ELISA respectively for anti-HIV antibodies and anti-TP antibody.The amount of positive and negative samples obtained by the two methods were taken for statistical analysis.Results： 400 samples were tested by the two methods respectively,and there was no difference between two methods through the test of chis-quare criterion.The coincidence of the results was 100%.Conclusion： Array-ELISA shows no statistical difference with ELISA kit,it may be a potential method for screening and detection in AIDS workstation.

关 键 词：微阵列酶联免疫法 酶联免疫吸附试验 人类免疫缺陷病毒 梅毒螺旋体 

分 类 号：R446.61[医药卫生—诊断学]