甲氧西林耐药表皮葡萄球菌的耐药基因检测及RAPD分型  

Drug-resistance genes detection and RAPD typing of methicillin-resistant Staphylococcus epidermidis

作　　者：林镯[1] 陈永平[1] 叶海油 阳韬[1] 陆小蒟[1] 陈少隆[1] 

机构地区：[1]温州医学院附属第一医院,浙江温州325000 [2]浙江省温州市鹿城区人民医院,浙江温州325000

出　　处：《中国卫生检验杂志》2011年第12期2989-2992,共4页Chinese Journal of Health Laboratory Technology

基　　金：温州市鹿城区科技计划项目(S090210)

摘　　要：目的:了解本地区耐甲氧西林表皮葡萄球菌(MRSE)的耐药基因存在状况,并对MRSE菌株进行基因同源性分型,为临床治疗及感染控制提供依据。方法:应用聚合酶链反应(PCR)对39株MRSE的6类耐药基因进行检测,并采用随机扩增多态性DNA(RAPD)技术对菌株进行基因同源性分型。结果:39株MRSE的mecA、ermB、ermC、msrA、tetM、tetK、Aac(6')/aph(2″)、aph(3')-III和mupA耐药基因的阳性率分别为100.0%(39/39)、12.8%(5/39)、94.9%(37/39)、43.6%(17/39)、5.1%(2/39)、2.6%(1/39)、82.1%(32/39)、25.6%(10/39)和12.8%(5/39),未检测出er-mA、tetO、tetL、ant(6')-I、vanA和vanB耐药基因;RAPD将39株MRSE分为11型,其中A型占48.7%(19/39)。结论:本地区的MRSE存在高比例的mecA、ermC、Aac(6')/aph(2″)耐药基因,RAPD分析提示MRSE的流行株以A型为主。Objective：To study the conditions of drug resistant genes and the homologous strains in methicillin-resistant Staphylococcus epidermidis（MRSE） in our region,in order to guide clinical treatment and infection control.Methods： Six type of resistant genes were detected by polymerase chain reaction（PCR） and homologous strains were typed by random amplified polymorphic DNA（RAPD） in the 39 MRSE strains.Results： The test of resistant genes in the 39 MRSE strains showed that occurrence of mecA,ermB,ermC,msrA,tetM,tetK,Aac（6′）/aph（2″）,aph（3′）-III and mupA were 100.0%（39/39）,12.8%（5/39）,94.9%（37/39）,43.6%（17/39）,5.1%（2/39）,2.6%（1/39）,82.1%（32/39）,25.6%（10/39） and 12.8%（5/39）,and the resistant genes of ermA,tetO,tetL,ant（6′）-I,vanA and vanB were negtive.The 39 MRSE strains were divided into 11 types by RAPD and type A accounted for 48.7%（19/39）.Conclusion： There were high percentage of mecA,ermC and Aac（6′）/aph（2″） resistant genes in MRSE and type A MRSE strains were the major prevalent strains in our region.

关 键 词：表皮葡萄球菌 甲氧西林耐药 耐药基因 随机扩增多态性DNA技术 

分 类 号：R378.11[医药卫生—病原生物学]