高效液相色谱法测定缬沙坦氢氯噻嗪分散片中两组分含量  

Determination of two components in Valsartan and Hydrochlorothiazide Dispersible Tablets by HPLC

作　　者：吴娟娟[1] 李玉琴[1] 

机构地区：[1]南通大学医学院,江苏南通226001

出　　处：《现代医药卫生》2011年第23期3532-3534,共3页Journal of Modern Medicine & Health

基　　金：南通大学博士科研启动基金(09R19)

摘　　要：目的：建立测定缬沙坦氢氯噻嗪分散片中缬沙坦、氢氯噻嗪含量的高效液相色谱法（high-performance liquid chromatog-raphy,HPLC）方法。方法：采用Shimadzu VP-ODS（4.6 mm×250 mm,5μm）色谱柱,以乙腈-0.1%磷酸溶液（用三乙胺调至pH4.0）（47：53）为流动相,检测波长271 nm,流速1.0 mL/min,柱温25℃,进样量20μL。结果 ：缬沙坦在32.05~160.25μg/mL（γ=0.9999）、氢氯噻嗪在5.05～25.25μg/mL（γ=0.9999）的范围内有良好的线性关系,平均回收率分别为98.7%（RSD=1.77%,n=9）、101.4%（RSD=1.94%,n=9）。结论：本方法简便、快速、准确,重复性好,可用于缬沙坦氢氯噻嗪分散片中两组分含量的测定。Objective：To establish a HPLC method for the determination of two components in Valsartan and Hydrochlorothiazide Dispersible Tablets.Methods：The concentrations were analyzed on the Shimadzu VP-ODS column（4.6 mm×250 mm,5 μm） with the mobile phase consisted of acetonitrile-0.1% phosphoric acid buffer solution（pH 4.0 adjusted by triethylamine）（53：47） at the detection wavelength of 271nm and the injection volume of 20 μL.The flow rate was 1.0 mL/min and the column temperature was 23 ℃.Results：Valsartan and hydrochlorothiazide had good linear relationship in the range of 32.05~160.25 μg/mL and 5.05~25.25 μg/mL.The average recoveries were 98.7%（RSD=1.77%,n=9） and 101.4%（RSD=1.94%,n=9）,respectively.Conclusion：This method is simple,fast and accurate,which can be applied to the concentration determination of Valsartan and Hydrochlorothiazide Dispersible Tablets.

关 键 词：高效液相色谱法 缬沙坦 氢氯噻嗪 缬沙坦氢氯噻嗪分散片 含量测定 

分 类 号：R9[医药卫生—药学]