大鼠前列腺增生模型阴茎海绵体内nNOS、eNOS表达研究  

Study on the alteration of nNOS, eNOS expression in corpus cavernosum of prostate hyperplasia model in rats

作　　者：金重睿[1] 俞建军[1] 徐月敏[1] 冯超[1] 叶绪晓[1] 

机构地区：[1]上海交通大学附属第六人民医院泌尿外科,上海200233

出　　处：《中国男科学杂志》2011年第10期12-16,共5页Chinese Journal of Andrology

摘　　要：目的分析引起阴茎勃起的主要神经递质NO合成的限速酶nNOS和eNOS在BPH模型大鼠阴茎海绵体中的表达。试探讨BPH引起ED的可能因素。方法20只雄性SD大鼠随机分为正常对照组和BPH模型组。通过去势后丙酸睾酮注射建立BPH模型，10只老年大鼠为老年对照组。4周后处死大鼠，分析不同组前列腺湿质量与前列腺指数，并在光镜下观察前列腺组织病理学改变。应用免疫组化方法研究不同组问大鼠阴茎海绵体内nNOS和eNOS阳性表达情况并比较组间差异。结果BPH模型组中前列腺湿质量和前列腺指数与正常对照组比较差异有统计学意义；显微镜下BPH模型组前列腺组织呈明显增生表现，大鼠BPH造模成功。免疫组化方法显示BPH模型组、老年对照组大鼠阴茎海绵体内的nNOS及eNOS阳性细胞表达率明显降低；nNOS及eNOS阳性表达与正常对照组的比较差异均有统计学意义。结论nNOS及eNOS是调控阴茎勃起的神经递质NO合成的关键酶，BPH模型组的阴茎海绵体内nNOS、eNOS表达明显减少或活性降低，导致NO释放减少可能是BPH引起勃起功能障碍的重要原因。Objective To investigate the alteration ofnNOS, eNOS expression in corpus cavernosum of rats with BPH, and explore possible relationship between BPH and erectile dysfunction. Methods Twenty adult Sprague-Dawley rats were randomly divided into experiment prostate hyperplasia model group and normal control group. The other 10 old rats were as the old control group. After 4 weeks, wet weight of prostate, the index of prostate, morphological and pathological changes were detected respectively. The expressions ofnNOS and eNOS in the cor- pus cavernosum were measured by immunohistochemistry. Results Compared with those of the normal control group, the wet weight and the index of the prostate were significantly increased in the experiment group. Remarkable prostate hyperplasia were observed by microscopy in the experiment group. The rat model of prostate hyperplasia was estab- lished successfully. The expressions of nNOS and eNOS in corpus cavernosum were significantly decreased in the experiment group and theold control group. There were differences in the expressions ofnNOS andeNOS between the experiment group, old control group and the normal control group; Whereas no differences in the expressions ofnNOS, eNOS were found between the experiment group and the old control group. Conclusion Penile erection is regulated by NO which is synthesized by nNOS andeNOS in rat penile tissues. Downregulation ofnNOS and eNOS in corpus cavernosum of rat with benign prostate hyperplasia might be a reason for erectile dysfunction.

关 键 词：前列腺增生 阴茎勃起 一氧化氮合酶: 大鼠模型 

分 类 号：R697.32[医药卫生—泌尿科学]