分散液相微萃取-高效液相色谱法测定水样中扑虱灵和哒螨灵  

HPLC Determination of Buprofezin and Pyridaben in Water Samples with Separation by Dispersion Liquid-Liquid Microextraction

作　　者：叶存玲[1] 刘清玲[1] 谢国红[2] 王治科[1] 

机构地区：[1]河南师范大学化学与环境科学学院,河南省环境污染控制重点实验室,新乡453007 [2]河南科技学院资源与环境学院,新乡453003

出　　处：《理化检验（化学分册）》2011年第12期1391-1394,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家自然科学基金项目(20977025);河南省教育厅自然科学基金项目(2009A150016);河南师范大学青年科学基金(2008qk12)

摘　　要：取水样置于离心管中,与含有三氯甲烷(萃取溶剂)的乙腈(分散剂)混合溶液充分混匀,静置5min,使水样中的扑虱灵和哒螨灵迅速富集于三氯甲烷液滴中,离心5min,下层三氯甲烷相经减压蒸干后,加入乙腈-水(75+25)混合溶液50μL溶解残渣,分取20μL溶液供高效液相色谱法分析。用Symmetry C18色谱柱作固定相,流动相为乙腈-水(75+25)混合溶液,检测波长为238nm。扑虱灵和哒螨灵的线性范围分别为2.0～100μg.L-1和1.0～100μg.L-1。回收率分别在81.2%～99.2%和87.3%～100.7%之间,相对标准偏差(n=5)分别在1.7%～8.3%及0.6%～9.1%之间。Two insecticides,buprofezin（BPFZ） and pyridaben（PRDB） were separated from water sample by mixing thoroughly with a mixture containing CH3CN and CHCl3 and standing for 5 min in a centrifuge tube.After centrifuging for 5 min,the solvent CHCl3 was removed by evaporation under reduced pressure and the residue was taken up with 50 μL of mixed solvent of CH3CN and water（75＋25）,from which an aliquot of 20 μL was taken for HPLC analysis.Symmetry C18 column was used as stantionary phase and mixture of CH3CN and water（75＋25） as mobile phase.UV detection at 238 nm was adopted in the determination.Linearity ranges were found between 2.0-100 μg·L-1（for BPFZ） and 1.0-100 μg·L-1（for PRDB）.Values of recovery and RSD′s（n=5） found were in the ranges of 81.2%-99.2%,87.3%-100.7%,and 1.7%-8.3%,0.6%-9.1% respectively.

关 键 词：高效液相色谱法 分散液-液微萃取 扑虱灵 哒螨灵 水样 

分 类 号：O657.7[理学—分析化学]