水稻矮杆突变体del1的生理特性及遗传分析  

PHYSIOLOGICAL CHARACTERISTICS AND GENETIC ANALYSIS OF RICE DWARF MUTANT del1

作　　者：韩瑞才 周平龙[1] 陈慧珍[1] 谭雪明[1] 石庆华[1] 潘晓华[1] 吴自明[1] 

机构地区：[1]江西农业大学/作物生理生态与遗传育种教育部重点实验室江西省作物生理生态与遗传育种重点实验室,江西南昌330045

出　　处：《核农学报》2011年第6期1077-1081,共5页Journal of Nuclear Agricultural Sciences

基　　金：国家"十二五"科技支撑计划项目(2011BAD16B04);国家自然科学基金(31060183);教育部博士点基金(20103603120001);江西省自然科学基金(2008GQN0019);江西省教育厅青年科学基金(GJJ09477)

摘　　要：利用60Coγ射线辐照粳稻品种Kitaake,获得一个性状稳定遗传的水稻矮杆突变体del1,本研究对其形态学、矮化性状遗传、叶片超微结构及生理特性进行了分析。结果表明该突变体田间表现为植株矮化,叶片直立,叶色浓绿和不结实;矮化突变性状由单隐性基因控制;突变体的叶肉细胞超微结构正常,但体积明显小于野生型;突变体幼苗株高对外源激素eBR钝感,对GA3较敏感;生育后期突变体保护酶活性较野生型下降较快。研究结果为该矮杆突变基因的定位克隆及功能分析奠定了基础。One rice dwarf mutant del1（dwarf and erected leaf）was isolated from a japonica rice variety Kitaake through60Co γ-rays irradiation inducement.Its characteristic of physiology,ultrastructure and genetic analysis were investigated.The results showed that del1 exhibited dwarf,dark green and erected leaves,and sterility.the dwarf phenotype was controlled by a single recessive nuclear gene.TEM analysis showed the ultrestructure of mutant del1 mesophyll cell was normal,but significant smaller than that of wild type.The mutant del1 was sensitive to GA3,and insensitive to eBR in terms of elongation of shoots.The protective enzyme activity of mutant leaf decreased more quickly than that of wild type.These results laid a basis for cloning and functional analysis of the dwarf gene.

关 键 词：水稻 突变体 矮秆 植物激素 衰老 

分 类 号：S511[农业科学—作物学]