寒兰株系间遗传多样性和亲缘关系的SSR分子标记分析  

ANALYSIS OF GENETIC DIVERSITY AND RELATIONSHIP OF Cymbidium kanran LINES USING SSR MARKERS

作　　者：徐晓薇[1] 江南[1] 杨俊波[2] 杨燕萍[1] 王丽芳[1] 

机构地区：[1]浙江省亚热带作物研究所,浙江温州325005 [2]中国科学院昆明植物研究所西南种质资源库,云南昆明650204

出　　处：《核农学报》2011年第6期1135-1141,共7页Journal of Nuclear Agricultural Sciences

基　　金：浙江省科技面上科研农业项目(2008C32016)

摘　　要：本研究筛选了从春兰和莲瓣兰中开发的7个SSR位点的引物,用于37个寒兰株系的遗传多样性和亲缘关系研究。研究结果表明,7个SSR位点的引物在寒兰中均具有非常好的通用性,而且每个位点在不同的株系中具有特有等位基因,特别是位点CSSR05、CSSR11和195。株系具有的特有等位基因可以用于株系鉴别。7个位点在寒兰株系间具有较高的遗传多样性,这可能与长期的人工选育有关。基于Nei氏遗传距离的聚类和分支分析表明,SSR标记可以用于寒兰株系间亲缘关系的分析。Seven SSR makers developed from Cymbidium goeringii and C.tortisepalum were used to investigate genetic diversity and relationship of Cymbidium kanran lines.The seven SSR markers had a promising transferability and application to the Cymbidium kanran lines.Each locus had specific alleles in different lines,especially the locus of CSSR05、CSSR11 and 195.The seven SSR markers revealed considerable genetic diversity from 37 C.kanran lines.It might be resulted from the longtime artificial breeding and hybridization.Clustering and cladistic analysis using Nei＇s genetic distance showed that SSR markers could be useful for genetic diversity analysis and variety identification.

关 键 词：寒兰 SSR标记 遗传多样性 株系鉴定 

分 类 号：S682.31[农业科学—观赏园艺]