基于悬浮细胞培养的半夏多倍体诱导研究:Ⅱ.单细胞水平COLO诱导及多倍体植株再生  

POLYPLOID INDUCED OF Pinellia ternata BASED ON SUSPENDED CELL CULTURE:Ⅱ.INDUCTION OF POLYPLOID CELL BASED ON SINGLE-CELLED LEVEL VIA COLO AND REGENERATION OF POLYPLOID PLANTS

作　　者：欧巧明[1] 岳春玲[2] 厚毅清[1] 崔文娟[1] 郑秀芳[3] 罗俊杰[1] 陈玉梁[1] 

机构地区：[1]甘肃省农业科学院生物技术研究所,甘肃兰州730070 [2]兰州大学第一医院药剂科,甘肃兰州730000 [3]河西学院农业与生物技术学院,甘肃张掖734000

出　　处：《核农学报》2011年第6期1148-1156,共9页Journal of Nuclear Agricultural Sciences

基　　金：国家转基因生物新品种培育重大专项(2009ZX08005-013B);甘肃省农科院农业科技创新专项(2011GAAS06-11);甘肃省农科院农业科技创新专项(2009GAAS16);甘肃省自然科学基金项目(0803RJZA041)

摘　　要：在建立了高效的半夏悬浮细胞培养和植株再生体系的基础上,本研究以悬浮细胞作为靶标材料,以秋水仙素(COLO)为多倍体诱导剂,对COLO诱导多倍体的适宜浓度等关键因素进行研究,并在获得多倍性细胞后,经诱导分化和再生形成纯合的多倍体植株。结果显示,0.05～0.10mg/ml的COLO处理24h,可获得较好的多倍体细胞诱导效果,多倍性细胞比率约为74.20%,并最终获得11株纯和的半夏多倍体幼苗,根尖细胞染色体为104条,是对照54条的2倍,目测诱变率可达14.07%～46.05%,多倍体诱导率为3.85%～29.76%,嵌合体率为12.88%～17.65%。结果证明通过半夏等植物单细胞水平多倍体诱导获得纯合的多倍体植株是一条可行且高效的多倍体诱导途径。Based on mature and efficient single-celled suspension cultivation and plant regeneration system,suspended cells were used as target material,key factors of polyploid induction via COLO,were studied in this paper.Homozygous polyploid plants were obtained via differentiation and regeneration culture after polyploid cell was obtained.The results showed that better induction efficiency of polyploid cell was obtained after suspended cells was treated with 0.05~0.10mg/ml COLO.Ratio of polyploid cell was about 74.20%.Eleven homozygous polyploid plants of Pinellia ternata was obtained finally and its cell chromosome number of root tip was 104 which was two times of control.Visual mutagenic rate was 14.07%~46.05% and induction rate of polyploid plants was 3.85%~29.76%,and rate of chimera was 12.88%~17.65%.The results proved that it was a viable and effective polyploid induction approach which suspended cell was induced into polyploidy cell via COLO and then differentiation and regeneration into homozygous polyploids.

关 键 词：半夏 悬浮细胞 单细胞水平 秋水仙素 多倍体 胚胎发生 植株再生 

分 类 号：S567.239[农业科学—中草药栽培]