高效液相色谱法测定黄芪桂枝五物颗粒中黄芪甲苷的含量  

Determination of astragaloside IV in Huangqi Guizhi Wuwu Granules by HPLC

作　　者：霍红[1] 李璐[1] 蔡航[1] 刘相辉[2] 杨一[2] 

机构地区：[1]武警黑龙江总队医院,哈尔滨150076 [2]黑龙江省哈尔滨市食品药品检验检测中心,150010

出　　处：《武警医学》2011年第12期1073-1074,1077,共3页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的建立控制黄芪桂枝五物颗粒质量的方法。方法采用C18色谱柱;流动相为乙腈一水(31:69);蒸发光散射检测器;柱温30℃。应用高效液相色谱法(high pelformance liquid chromatography,HPLC)测定黄芪桂枝五物颗粒中黄芪甲苷的含量。结果黄芪甲苷在2～10μg范围内与峰面积呈良好的线性关系(r=0.9990,n=5);重复性试验RSD值为1.6%(n=6);平均回收率为97.91%,RSlD为1.23%(n=6);供试品溶液在8 h内稳定。结论该方法准确、可靠、重现性好,可作为黄芪桂枝五物颗粒的质量控制指标。Objective To establish an HPLC method for the determination of astragaloside Ⅳ in Huangqi Guizhi Wuwu Granules. Methods The chromatographic system consisted of C18 column with acetonitrilewater（31： 69） as the mobile phase. ELSD was used. The temperature of the column was 30 ℃. Results Astragaloside Ⅳ showed good linearity （ r = 0. 9990） within the range of 2 - l0 μg. the RSD of repeatability test was 1.6% （n =6 ）. The average recovery was 97.91% with RSD = 1.23% （n = 6）. The sample solution was stable in eight hours. Conclusions This method is accurate, reliable and reproducible , which can be used for quality control of Huangqi Guizhi Wuwu Granules.

关 键 词：黄芪桂枝五物颗粒 黄芪甲苷 高效液相色谱法 

分 类 号：R286.0[医药卫生—中药学]