白念珠菌pH应答转录因子Rim101p在大肠杆菌中的表达  

The Expression of Candida albcians Transcirptial Factor Rim101p in E. coli

作　　者：桂磊[1] 郑雯[1] 邢来君[1] 李明春[1] 

机构地区：[1]南开大学微生物学系分子微生物学与技术教育部重点实验室,天津300071

出　　处：《南开大学学报（自然科学版）》2011年第5期96-100,共5页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家自然科学基金(31070126;81171541);天津市应用基础及前沿技术研究计划(10JCYBJC09700)

摘　　要：Rim101蛋白是白念珠菌中pH应答反应的重要转录因子.为进一步研究Rim101p的功能,采用PCR方法获得RIM101基因序列,并将其克隆入表达载体pET-21a,转化大肠杆菌表达菌BL21,通过摸索诱导条件使Rim101p在大肠杆菌中成功表达.EMSA实验结果进一步验证了重组Rim101p的活性.In Candida albicans,Rim101p is an important transcripital factor to pH response.For further research on the function of Rim101p,Rim101 gene was cloned,expression voctor pET-21a-Rim101 was constructed and Rim101p express in E.coli was made successfully.EMSA results suggest that Rim101p expressed in E.coli had the ability to bind GCCAAGAA element.

关 键 词：白念珠菌 Rim101p EMSA 

分 类 号：Q78[生物学—分子生物学]