气相色谱/质谱联用法测定全血中的五种酰胺类除草剂  

The Simultaneous Analysis of Five Acyl-amine Herbicides in Whole Blood by GC/MS

作　　者：杨士云[1] 乔静[1] 闫志伟[1] 

机构地区：[1]北京市公安局法医检验鉴定中心,北京100192

出　　处：《现代科学仪器》2011年第6期138-140,共3页Modern Scientific Instruments

摘　　要：目的:建立血液中五种酰胺类除草剂的气相色谱/质谱联用分析方法。方法:采用环己烷液液提取,气相色谱/质谱联用法定性定量。结果:五种酰胺类除草剂与血中杂质分离良好,回收率分别为:甲草胺80.5%,乙草胺81.8%,丙草胺83.6%,丁草胺84.2%,异丙草胺85.03%,最低检出限分别为:甲草胺50ng/mL,乙草胺150ng/mL,丙草胺50ng/mL,丁草胺50ng/mL,异丙草胺50ng/mL,五种除草剂在0.5～10μg/mL之间均呈良好的线性关系,相关系数r>0.999。结论:该方法操作简单快速,重现性好,适合于刑事案件中此类药物的定性定量分析。Objective：An analysis method of five acyl-amine herbicides in whole blood by GC/MS is established.Methods：That herbicides were extrated from blood using cycl ohexane,qualitative and quantitative analysis was obtained by GC/MS.Results：The five herbicides can be separated from impurities in blood.The recoveries for Alachlor,Acetochlor,Pretilachlor,Butachlor,Metolachlor were 80.5%,81.8%,83.6%,84.2%,85.03% respectively.The limits of qualification for Alachlor,Acetochlor,Pretilachlor,Butachlor,Metolachlor were 50ng/mL,150ng/mL,50ng/mL,50ng/mL,50ng/mL respectively.The correlation coefficient of linear calibration curve was over 0.999 within concentration range 0.5μg/mL ~20μg/mL.Conclusion：The method was simple,rapid and accurate for qualitative and quantitative analysis of this five herbicides.

关 键 词：法医毒物分析 除草剂 酰胺类 气相色谱/质谱联用 

分 类 号：D919.2[医药卫生—法医学] O657.63[政治法律—法学]